Gustatory habituation to essential oil induces reduced feeding deterrence and neuronal desensitization in Spodoptera litura

IF 4.3 1区 农林科学 Q1 ENTOMOLOGY
Hyoeun Jeon, Jun-Hyung Tak
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Abstract

The tobacco cutworm, Spodoptera litura, is one of the most important agricultural insect pests. Plant essential oils can be considered potential candidates for eco-friendly control agents, as they exhibit insecticidal and feeding deterrent activity. The present study investigated the antifeedant and insecticidal activity of 29 essential oils. Their potential for habituation and association with the gustatory sensilla were also examined. In no-choice tests and contact-fumigation bioassays on third instar larvae, clove bud, fennel sweet, and lemongrass oils exhibited notable activities. Still, no direct correlation between insecticidal activity and feeding deterrence was observed. Second instar larvae were pre-exposed to those active oils to test the habituation effect. Larvae reared with lemongrass and clove bud oils showed gustatory habituation, whereas those with fennel sweet oil did not show any desensitization compared to the control. Comparable outcomes were observed in individuals exposed to the main constituents of the three oils. Additionally, the mixture of fennel sweet and clove bud oils showed a synergistic feeding deterrent effect. However, although statistically insignificant, potential habituation for the mixture was observed, and only robust inhibition of habituation was expected at physiologically high concentrations (FDI90 + FDI90). Electrophysiological studies showed that the response of the maxillary palp to citral decreased in the experienced group, while to trans-anethole, it was maintained at levels similar to the naive group. The reduction in feeding deterrence corresponded to the repeated exposure and desensitization of the maxillary palp, varying with the types of essential oils.

Abstract Image

对香精油的味觉习惯会降低鞘翅目昆虫的摄食阻遏和神经元脱敏作用
烟草切割虫(Spodoptera litura)是最重要的农业害虫之一。植物精油具有杀虫和阻止取食的活性,可被视为生态友好型控制剂的潜在候选物质。本研究调查了 29 种植物精油的反食性和杀虫活性。同时还考察了它们的习性潜力以及与味觉感受器的关联。在对三龄幼虫进行的无选择试验和接触熏蒸生物测定中,丁香芽、茴香甜和柠檬草精油表现出了显著的活性。不过,并未观察到杀虫活性与摄食阻遏之间的直接关联。二龄幼虫预先接触了这些活性油,以测试习惯效应。与对照组相比,用柠檬草油和丁香芽油饲养的幼虫表现出了味觉习惯,而用茴香甜油饲养的幼虫则没有表现出任何脱敏现象。在接触这三种油的主要成分的个体中也观察到了类似的结果。此外,茴香甜油和丁香芽油的混合物还显示出协同的摄食阻吓效果。不过,尽管在统计学上并不显著,但还是观察到了混合物的潜在习性,而且只有在生理浓度较高(FDI90 + FDI90)时才会对习性产生强有力的抑制作用。电生理学研究表明,有经验组的上颚对柠檬醛的反应降低,而对反式茴香醚的反应则维持在与无经验组相似的水平。摄食阻吓的降低与反复接触和上颚脱敏有关,因精油的种类而异。
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来源期刊
Journal of Pest Science
Journal of Pest Science 生物-昆虫学
CiteScore
10.40
自引率
8.30%
发文量
114
审稿时长
6-12 weeks
期刊介绍: Journal of Pest Science publishes high-quality papers on all aspects of pest science in agriculture, horticulture (including viticulture), forestry, urban pests, and stored products research, including health and safety issues. Journal of Pest Science reports on advances in control of pests and animal vectors of diseases, the biology, ethology and ecology of pests and their antagonists, and the use of other beneficial organisms in pest control. The journal covers all noxious or damaging groups of animals, including arthropods, nematodes, molluscs, and vertebrates. Journal of Pest Science devotes special attention to emerging and innovative pest control strategies, including the side effects of such approaches on non-target organisms, for example natural enemies and pollinators, and the implementation of these strategies in integrated pest management. Journal of Pest Science also publishes papers on the management of agro- and forest ecosystems where this is relevant to pest control. Papers on important methodological developments relevant for pest control will be considered as well.
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