{"title":"The value of urinary exosomal microRNA-21 in the early diagnosis and prognosis of bladder cancer.","authors":"Fu-Kan Yang, Chao Tian, Lin-Xiong Zhou, Tian-Yu Guan, Gui-Liu Chen, Yi-Ying Zheng, Zheng-Guo Cao","doi":"10.1002/kjm2.12845","DOIUrl":null,"url":null,"abstract":"<p><p>Bladder cancer (BC) poses high morbidity and mortality, with urinary exosomal microRNA (miR)-21 showing potential value in its diagnosis and prognosis, and we probed its specific role. We prospectively selected 116 BC patients and 116 healthy volunteers as the BC and control groups, respectively. BC urinary exosomal miR-146a-5p, miR-93-5p, miR-663b, miR-21, and miR-4454 relative expression levels were assessed. The correlations between clinical indexes and urinary exosomal miR-21, prognostic value of miR-21, and diagnostic value of the five candidate miRNAs, urine cytology, and miRNA joint diagnostic panel for BC and urinary exosomal miR-21, miR-4454, and urine cytology for Ta-T1 and T2-T4 stage BC were analyzed. Urinary exosomal miR-146a-5p, miR-93-5p, miR-663b, miR-21, and miR-4454 were highly expressed in BC patients. miR-146a-5p, miR-93-5p, miR-663b, miR-21, miR-4454, miRNA combined diagnostic panel, and urine cytology had certain diagnostic value for BC, with miR-21, miR-4454, and miRNA co-diagnostic panel showing the highest diagnostic value. Collectively, urinary exosomal miR-21 was closely related to Tumor-Node-Metastasis staging and grading in BC patients. Urinary exosomal miR-21 had high diagnostic value for BC and Ta-T1 and T2-T4 stage BC, and had high predictive value for BC poor prognosis, providing an effective indicator for the occurrence, development, and prognostic assessment of BC.</p>","PeriodicalId":94244,"journal":{"name":"The Kaohsiung journal of medical sciences","volume":" ","pages":"660-670"},"PeriodicalIF":0.0000,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Kaohsiung journal of medical sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/kjm2.12845","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/27 0:00:00","PubModel":"Epub","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Bladder cancer (BC) poses high morbidity and mortality, with urinary exosomal microRNA (miR)-21 showing potential value in its diagnosis and prognosis, and we probed its specific role. We prospectively selected 116 BC patients and 116 healthy volunteers as the BC and control groups, respectively. BC urinary exosomal miR-146a-5p, miR-93-5p, miR-663b, miR-21, and miR-4454 relative expression levels were assessed. The correlations between clinical indexes and urinary exosomal miR-21, prognostic value of miR-21, and diagnostic value of the five candidate miRNAs, urine cytology, and miRNA joint diagnostic panel for BC and urinary exosomal miR-21, miR-4454, and urine cytology for Ta-T1 and T2-T4 stage BC were analyzed. Urinary exosomal miR-146a-5p, miR-93-5p, miR-663b, miR-21, and miR-4454 were highly expressed in BC patients. miR-146a-5p, miR-93-5p, miR-663b, miR-21, miR-4454, miRNA combined diagnostic panel, and urine cytology had certain diagnostic value for BC, with miR-21, miR-4454, and miRNA co-diagnostic panel showing the highest diagnostic value. Collectively, urinary exosomal miR-21 was closely related to Tumor-Node-Metastasis staging and grading in BC patients. Urinary exosomal miR-21 had high diagnostic value for BC and Ta-T1 and T2-T4 stage BC, and had high predictive value for BC poor prognosis, providing an effective indicator for the occurrence, development, and prognostic assessment of BC.
膀胱癌(BC)的发病率和死亡率都很高,尿液外泌体microRNA(miR)-21在其诊断和预后方面具有潜在价值,我们对其特殊作用进行了探究。我们前瞻性地选择了 116 名 BC 患者和 116 名健康志愿者分别作为 BC 组和对照组。我们评估了 BC 尿液外泌体 miR-146a-5p、miR-93-5p、miR-663b、miR-21 和 miR-4454 的相对表达水平。分析了临床指标与尿液外泌体miR-21之间的相关性、miR-21的预后价值、五种候选miRNA、尿液细胞学和miRNA联合诊断面板对BC的诊断价值,以及尿液外泌体miR-21、miR-4454和尿液细胞学对Ta-T1和T2-T4期BC的诊断价值。尿液外泌体miR-146a-5p、miR-93-5p、miR-663b、miR-21和miR-4454在BC患者中高表达。miR-146a-5p、miR-93-5p、miR-663b、miR-21、miR-4454、miRNA联合诊断小组和尿液细胞学检查对BC有一定的诊断价值,其中miR-21、miR-4454和miRNA联合诊断小组的诊断价值最高。总之,尿液外泌体miR-21与BC患者的肿瘤-结节-转移分期和分级密切相关。尿液外泌体miR-21对BC、Ta-T1和T2-T4期BC有很高的诊断价值,对BC不良预后有很高的预测价值,为BC的发生、发展和预后评估提供了一个有效的指标。