Examination of cytotoxic and antimicrobial effect of whitening toothpastes: an in vitro study.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-27 DOI:10.2340/aos.v83.40774

Numan Aydın, Aysun Kılıç Süloğlu, Neslihan İdil, Selen Öztürk, Serpil Karaoğlanoğlu

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引用次数: 0

Abstract

Objective: Toothpastes are widely used to protect oral and teeth health. This study aims to examine the cytotoxic and antimicrobial effects of whitening toothpastes.

Methods: In this study, extracts were prepared according to ISO 10993-12:2021 standard (0.2 g/mL) using whitening and conventional toothpastes. The prepared extracts were added to human gingival fibroblast cell lines (HGF-1) in different dilutions (1:1, 1:2, 1:4, 1:8, 1:16, and 1:32) and a cytotoxicity test was performed. Antimicrobial analysis of toothpastes was performed on Streptococcus mutans, Staphylococcus aureus, and Candida albicans using the hole-plate diffusion method. Cell viability and microbial analysis data were examined using two-way analysis of variance (ANOVA) and Tukey post-hoc test (p < 0.05).

Results: Toothpastes with sodium lauryl sulfate (SLS) in their composition showed statistically more toxic effects (p < 0.05). The activated carbon toothpastes without SLS showed over 90% cell viability after dilution. Although the dilution rate of toothpastes containing SLS increased, cell viability remained below 70%. All toothpastes used in the study showed antimicrobial effects on S. mutans, S. aureus, and C. albicans. Toothpaste containing hydrogen peroxide and SLS produced more antibacterial effects than activated carbon, blue covarine, microparticles, and conventional toothpaste.

Conclusions: SLS-containing toothpastes showed more toxicity on HGF-1 cells. Toothpaste containing hydroxyapatite did not show toxic effects on HGF-1 cells. SLS, sodium lauryl sarcosinate and hydrogen peroxide in toothpastes increase antimicrobial effects.

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检测美白牙膏的细胞毒性和抗菌效果：一项体外研究。

目的牙膏被广泛用于保护口腔和牙齿健康。本研究旨在探讨美白牙膏的细胞毒性和抗菌作用：本研究根据 ISO 10993-12:2021 标准（0.2 克/毫升），使用美白牙膏和传统牙膏制备提取物。将制备好的提取物以不同的稀释度（1:1、1:2、1:4、1:8、1:16 和 1:32）添加到人牙龈成纤维细胞系（HGF-1）中，并进行细胞毒性测试。使用孔板扩散法对牙膏中的变异链球菌、金黄色葡萄球菌和白色念珠菌进行了抗菌分析。细胞活力和微生物分析数据采用双向方差分析（ANOVA）和Tukey事后检验（P < 0.05）进行检验：结果：从统计学角度看，含有十二烷基硫酸钠（SLS）的牙膏毒性更强（p < 0.05）。不含 SLS 的活性炭牙膏在稀释后的细胞存活率超过 90%。虽然含有 SLS 的牙膏稀释率增加，但细胞存活率仍低于 70%。研究中使用的所有牙膏都对突变菌、金黄色葡萄球菌和白喉杆菌有抗菌作用。含有过氧化氢和 SLS 的牙膏比活性炭、蓝花楹、微颗粒和传统牙膏产生更多的抗菌效果：结论：含 SLS 的牙膏对 HGF-1 细胞的毒性更大。结论：含 SLS 的牙膏对 HGF-1 细胞的毒性更大，而含羟基磷灰石的牙膏对 HGF-1 细胞没有毒性作用。牙膏中的 SLS、十二烷基肌氨酸钠和过氧化氢会增加抗菌效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.