Development of an HPLC device with water/acetonitrile with NaCl mixed solution that induces a phase-separation multiphase flow as the eluent in the separation column

IF 1.8 4区化学 Q3 CHEMISTRY, ANALYTICAL

Analytical Sciences Pub Date : 2024-05-26 DOI:10.1007/s44211-024-00599-3

Ryuto Hirose, Takeshi Iharada, Kazuhiko Tsukagoshi

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Abstract

We developed a novel HPLC device where the phase-separation multiphase flow worked as the eluent in the separation column by using a water/acetonitrile/ethyl acetate triple mixed solution as a dual-phase-separation solution. Dual-phase-separation solutions form a phase-separation multiphase flow in a microscopic space. The new separation mechanism in the HPLC is called phase-separation mode. In this study, we used water and acetonitrile with NaCl mixed solution as a dual-phase-separation solution instead of the triple mixed solution. Octadecylsilyl (ODS)-modified particle- and porous silica particle-packed separation columns were united with the HPLC device for phase-separation mode caused by phase-separation multiphase flow. NA (1-naphthol) and NDS (2,6-naphthalenedisulfonic acid) were analyzed by the device as model sample. Using the water and acetonitrile with NaCl mixed solution at the solvent volume ratio of 5:5, NA and NDS were not separated on either column at 25 °C. On the other hand, they were separated with the order NDS and NA on the ODS column and separated with the order NA and NDS on the silica column in phase-separation mode at 0 °C. We discuss the separation mechanism of phase-separation mode using the water and acetonitrile with NaCl mixed solution at 0 °C.

Graphical abstract

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开发出一种高效液相色谱装置，采用水/乙腈加氯化钠混合溶液作为分离柱中的洗脱液，从而产生相分离多相流。

我们利用水/乙腈/乙酸乙酯三合一混合溶液作为双相分离溶液，开发了一种新型高效液相色谱装置，在分离柱中使用相分离多相流作为洗脱液。双相分离溶液在微观空间中形成相分离多相流。高效液相色谱中的新分离机制称为相分离模式。在本研究中，我们使用水和乙腈加氯化钠混合溶液作为双相分离溶液，而不是三相混合溶液。十八烷基硅烷（ODS）改性颗粒和多孔硅胶颗粒填料分离柱与高效液相色谱装置相结合，实现了相分离多相流的相分离模式。该装置以 NA（1-萘酚）和 NDS（2,6-萘二磺酸）为样品进行分析。在 25 °C 下，使用水和乙腈加氯化钠的混合溶液（溶剂体积比为 5:5），NA 和 NDS 在两个色谱柱上均未分离。另一方面，在 ODS 色谱柱上，它们按 NDS 和 NA 顺序分离；在 0 °C 时，在相分离模式下，在硅胶色谱柱上，它们按 NA 和 NDS 顺序分离。我们讨论了在 0 °C 下使用水和乙腈加氯化钠混合溶液进行相分离模式的分离机理。

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来源期刊

Analytical Sciences 化学-分析化学

CiteScore

2.90

自引率

18.80%

发文量

232

审稿时长

1 months

期刊介绍： Analytical Sciences is an international journal published monthly by The Japan Society for Analytical Chemistry. The journal publishes papers on all aspects of the theory and practice of analytical sciences, including fundamental and applied, inorganic and organic, wet chemical and instrumental methods. This publication is supported in part by the Grant-in-Aid for Publication of Scientific Research Result of the Japanese Ministry of Education, Culture, Sports, Science and Technology.