Recent advancements in microfluidic-based biosensors for detection of genes and proteins: Applications and techniques

IF 10.61 Q3 Biochemistry, Genetics and Molecular Biology

Biosensors and Bioelectronics: X Pub Date : 2024-05-22 DOI:10.1016/j.biosx.2024.100489

Saeed Siavashy , M. Soltani , Shayan Rahimi , Mehraveh Hosseinali , Zahra Guilandokht , Kaamran Raahemifar

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Abstract

This article has tried to provide an overview of the most sophisticated microfluidic biosensors for identifying nucleic acids and proteins at the site of treatment. Microfluidics, which also automates sample preparation and reduces processing time and reagent consumption, enables the analysis of small sample quantities. Microfluidics and biosensor technologies collaborate to provide diagnostics at the point of care with high throughput analysis, portability, and disposability. The high sensitivity and selectivity requirements, false response errors, and integration with other essential modules are some of the challenges posed by this merger. The broad categories of protein-based and DNA-based biosensor technology are covered in this review. Also, recent advancements in coupling the biosensors to microfluidics, the main challenges and potential solutions in deploying microfluidic biosensors for point-of-care diagnostics, and the most recent developments in these areas have been discussed.

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用于检测基因和蛋白质的微流控生物传感器的最新进展：应用与技术

本文试图概述用于鉴定治疗部位核酸和蛋白质的最先进的微流控生物传感器。微流控技术还能自动进行样品制备，减少处理时间和试剂消耗，从而实现对少量样品的分析。微流控技术与生物传感器技术相结合，可在医疗点提供诊断服务，具有高通量分析、便携性和可抛弃性等特点。高灵敏度和高选择性要求、错误响应误差以及与其他重要模块的整合是这一合并带来的部分挑战。本综述涵盖了基于蛋白质和 DNA 的生物传感器技术两大类。此外，还讨论了将生物传感器与微流控技术相结合的最新进展、将微流控生物传感器用于床旁诊断的主要挑战和潜在解决方案，以及这些领域的最新发展。

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来源期刊

Biosensors and Bioelectronics: X Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

4.60

自引率

0.00%

发文量

166

审稿时长

54 days

期刊介绍： Biosensors and Bioelectronics: X, an open-access companion journal of Biosensors and Bioelectronics, boasts a 2020 Impact Factor of 10.61 (Journal Citation Reports, Clarivate Analytics 2021). Offering authors the opportunity to share their innovative work freely and globally, Biosensors and Bioelectronics: X aims to be a timely and permanent source of information. The journal publishes original research papers, review articles, communications, editorial highlights, perspectives, opinions, and commentaries at the intersection of technological advancements and high-impact applications. Manuscripts submitted to Biosensors and Bioelectronics: X are assessed based on originality and innovation in technology development or applications, aligning with the journal's goal to cater to a broad audience interested in this dynamic field.