Monoclonal antibody-based enzyme-linked immunosorbent assay for quantification of majonoside R2 as an authentication marker for Nngoc Linh and Lai Chau ginsengs

IF 6.8 2区 医学 Q1 CHEMISTRY, MEDICINAL
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Abstract

Background

Recent years have witnessed increasing interest in the high amount of ocotillol-type saponin in Panax vietnamensis, particularly in relation to majonoside R2 (MR2). This unique 3%–5% MR2 content impart Ngoc Linh and Lai Chau ginsengs with unique pharmacological activities. However, in the commercial domain, unauthentic species have infiltrated and significantly hindered access to the authentic, efficacious variety. Thus, suitable analytical techniques for distinguishing authentic Vietnamese ginseng species from others is becoming increasingly crucial. Therefore, MR2 is attracting considerable attention as a target requiring effective management measures.

Methods

An enzyme-linked immunosorbent assay (ELISA) was developed by producing monoclonal antibodies against MR2 (mAb 16E11). The method was thoroughly validated, and the potential of the immunoassay was confirmed by high-performance liquid chromatography with ultraviolet spectroscopy. Furthermore, ELISA was applied to the assessment of the MR2 concentrations of various Panax spp., including Korean, American, and Japanese ginsengs.

Results and conclusions

An icELISA using mAb 16E11 exhibited linearity between 3.91 and 250 ng/mL of MR2, with detection and quantification limits of 1.53 and 2.50 46.6 ng/mL, respectively. Based on this study, the developed icELISA using mAb 16E11 could be a valuable tool for analyzing MR2 level to distinguish authentic Ngoc Linh and Lai Chau ginsengs from unauthentic ones. Furthermore, the analysis of the samples demonstrated that Ngoc Linh and Lai Chau ginsengs exhibit a notably higher MR2 value than all other Panax spp. Thus, MR2 might be their ideal marker compound, and various bioactivities of this species should be explored.

Abstract Image

基于单克隆抗体的酶联免疫吸附测定法,用于定量检测作为玉莲和莱州人参鉴别标志的雄花苷 R2
背景近年来,人们对越南人参中的大量乌头醇类皂苷,特别是马钱子苷 R2(MR2)越来越感兴趣。3%-5% 的独特 MR2 含量赋予了玉莲和莱州人参独特的药理活性。然而,在商业领域,非正宗人参品种已经渗透进来,严重阻碍了人们获得正宗、有效的人参品种。因此,采用合适的分析技术来区分正宗越南人参品种变得越来越重要。方法通过生产针对 MR2 的单克隆抗体(mAb 16E11),开发了一种酶联免疫吸附测定法(ELISA)。对该方法进行了全面验证,并通过高效液相色谱法和紫外光谱法证实了该免疫测定法的潜力。结果与结论使用 mAb 16E11 的 icELISA 在 3.91 至 250 纳克/毫升的 MR2 之间呈线性关系,检出限和定量限分别为 1.53 和 2.50 - 46.6 纳克/毫升。根据这项研究,使用 mAb 16E11 开发的 icELISA 是分析 MR2 含量的重要工具,可用于区分玉莲和莱州人参的真假。此外,对样品的分析表明,玉莲和莱州人参的 MR2 值明显高于所有其他三七属植物。因此,MR2 可能是其理想的标记化合物,应探索该物种的各种生物活性。
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来源期刊
Journal of Ginseng Research
Journal of Ginseng Research CHEMISTRY, MEDICINAL-INTEGRATIVE & COMPLEMENTARY MEDICINE
CiteScore
11.40
自引率
9.50%
发文量
111
审稿时长
6-12 weeks
期刊介绍: Journal of Ginseng Research (JGR) is an official, open access journal of the Korean Society of Ginseng and is the only international journal publishing scholarly reports on ginseng research in the world. The journal is a bimonthly peer-reviewed publication featuring high-quality studies related to basic, pre-clinical, and clinical researches on ginseng to reflect recent progresses in ginseng research. JGR publishes papers, either experimental or theoretical, that advance our understanding of ginseng science, including plant sciences, biology, chemistry, pharmacology, toxicology, pharmacokinetics, veterinary medicine, biochemistry, manufacture, and clinical study of ginseng since 1976. It also includes the new paradigm of integrative research, covering alternative medicinal approaches. Article types considered for publication include review articles, original research articles, and brief reports. JGR helps researchers to understand mechanisms for traditional efficacy of ginseng and to put their clinical evidence together. It provides balanced information on basic science and clinical applications to researchers, manufacturers, practitioners, teachers, scholars, and medical doctors.
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