DEVELOPMENT OF A METHOD FOR DETERMINING SERTRALINE IN THE BLOOD

Abstract

Aim Development of methods for isolation and determination of sertraline in biological fluids. Materials and methods. The study was conducted with tablettes "Zoloft" ("Pfizer", USA) using the following equipment: liquid chromatograph Shimadzu LC-20 Prominence (Japan) with detector SPD-M20A, gas chromatograph Agilent Technologies (USA) 7890 A / 5977 MSD, (program MassHunter GC-MS ), waters vacuum unit and cartridges for solid-phase extraction Oasis HLB; papain enzymes (MedFloran, Russia); trypsin, chymotrypsin, hyaluronidase (Lidaza) (Samson-Med LLC, Russia); test strips from different manufacturers under trade names: "Be sure", "NarcoCHEC", "FACTOR-MED". Urine samples were obtained after administration of the drug to laboratory animals - guinea pigs. Isolation of the base of sertraline from a solution of its salt was carried out by LLE with organic solvents and their mixtures at pH = 10, 11 and 12 (pKa sertraline 9.48). Obtaining data on the effectiveness of blood sample preparation methods (liquid-liquid extraction, solid-phase extraction and enzymatic hydrolysis) for sertraline was carried out on enriched model samples of donor blood according to the method proposed by Choger. Results. Cross-positive urine test results were obtained with immunochromatographic test strips for derivatives of e 1,4-benzodiazepine and synthetic cannabimimetics ("spice"). And a follow-up with 1 mg / ml solution of sertraline gave a negative result. Desmethylsertraline, a major metabolite, was determined in the urine, a peak with a retention time of about 12.75 min, the peak of the original substancesand during the synthesis of sertraline - sertralinimine and the peak of the native sertraline and with a retention time of about 12.65 minutes. A method for detecting sertraline by HPLC has been developed. Conclusion. The use of the technique of enzymatic hydrolysis of blood with trypsin allows to increase the degree of extraction by 1.5 times compared with liquid-liquid extraction with chloroform and by 2 times compared with solid-phase extraction.