Evaluating the Antimicrobial Activity of Esomeprazole Magnesium Trihydrate on Antibiotic-Resistant Bacteria Using Efflux Pumps

Qeios Pub Date : 2024-05-20 DOI:10.32388/aq4wlb

Thualfakar Hayder Hasan Abusaiba

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Abstract

BACKGROUND: Antibiotic resistance mediated by efflux pumps is a serious public health threat. Esomeprazole magnesium trihydrate (EMT) is a proton pump inhibitor with reported antimicrobial activity, but its effects against efflux-mediated resistance are unknown. OBJECTIVE: To evaluate the antimicrobial activity of EMT alone and in combination with the efflux pump inhibitor reserpine against clinical isolates of _Pseudomonas aeruginosa, Acinetobacter baumannii_ and _Staphylococcus aureus_. METHODS: Minimum inhibitory concentration (MIC) assays were performed using the broth microdilution method on 15 non-duplicate clinical isolates and reference strains with/without EMT and reserpine. RESULTS: EMT demonstrated activity against all isolates, with MIC ranges of 4-32 μg/mL, 8-64 μg/mL and 2-16 μg/mL respectively. EMT MICs decreased 4-8-fold for 13/15 isolates when combined with reserpine, indicating EMT may inhibit efflux pumps. Similar reductions occurred for comparator antibiotics. CONCLUSIONS: This study provides the first evidence that EMT possesses intrinsic antimicrobial activity against these pathogens and may function as both an efflux pump substrate and inhibitor. EMT warrants further investigation as a potential adjuvant antibiotic for overcoming efflux-mediated resistance.

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利用外排泵评估埃索美拉唑镁三水合物对耐药细菌的抗菌活性

背景：由外排泵介导的抗生素耐药性是一种严重的公共卫生威胁。目的：评估 EMT 单独使用以及与外排泵抑制剂瑞瑟平联合使用对铜绿假单胞菌、鲍曼不动杆菌和金黄色葡萄球菌临床分离株的抗菌活性。方法：采用肉汤微稀释法对 15 个不重复的临床分离株和参考菌株进行了最低抑菌浓度（MIC）测定，结果表明：EMT 对所有分离株都有活性，MIC 范围分别为 4-32μg/mL、8-64 μg/mL 和 2-16μg/mL。当 EMT 与利血平合用时，13/15 株分离物的 EMT MIC 下降了 4-8 倍，这表明 EMT 可能抑制了外排泵。对比抗生素也有类似的降低：本研究首次证明了 EMT 对这些病原体具有内在抗菌活性，并可同时作为外排泵底物和抑制剂发挥作用。EMT 作为克服外排介导的耐药性的潜在辅助抗生素，值得进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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