Polymorphism and resistance spectrum to Magnaporthe oryzae analysis of Pi-d2 haplotypes in rice (Oryza sativa L.) resource from Yunnan province of China

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-22 DOI:10.1017/s1479262124000248

Yi Yang, Jinbin Li, Jiqiong Ma, Jing Tao, Chengpeng Li, Yiding Sun, Minghui Xu

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Abstract

Pi-d2, which encodes a potential serine-threonine receptor-like kinase (RLK) membrane-spanning protein consisting of 825 amino acids, confers resistance to Magnaporthe oryzae strain ZB15 via an unidentified recognition mechanism. In this study, the Pid2 alleles of 303 rice (O. sativa) varieties from China's Yunnan region were amplified and sequenced in order to produce 24 haplotypes and 16 translation variants. Six of twenty-four alleles possessing the resistant site at the 441st amino acid were chosen for evaluating blast resistance by transforming into the blast-vulnerable rice variety Nipponbare. After being infected with 11 strains of M. oryzae, all transgenic lines exhibited resistance to ZB-15, whereas resistance to other strains varied. Notably, Pi-d2_H23 and Pi-d2_H24 exhibited resistance to all M. oryzae strains tested, indicating that these two alleles may have a broader resistance spectrum to M. oryzae. Alignment of these alleles’ amino acid sequences revealed that the differences in blast resistance spectra were primarily related to the amino acids present in the PAN domain at position 363 (valine/alanine). These findings suggested that the two extracellular signal recognition domains of PI-D2, B-lectin and PAN, may play a role in the identification of M. oryzae effectors. The present results provide insight into the mechanism of interaction between RLKs and M. oryzae.

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中国云南省水稻（Oryza sativa L.）资源中 Pi-d2 单倍型的多态性及对 Magnaporthe oryzae 的抗性谱分析

Pi-d2编码一种潜在的丝氨酸-苏氨酸受体样激酶（RLK）跨膜蛋白，由825个氨基酸组成，通过一种未确定的识别机制赋予其对木格氏球菌（Magnaporthe oryzae）菌株ZB15的抗性。本研究对中国云南地区 303 个水稻（O. sativa）品种的 Pid2 等位基因进行了扩增和测序，以产生 24 个单倍型和 16 个翻译变体。从 24 个等位基因中选择了 6 个在第 441 个氨基酸处具有抗性位点的等位基因，将其转化到易感稻瘟病的水稻品种 "日本稻 "中，以评估其稻瘟病抗性。在感染 11 株 M. oryzae 后，所有转基因品系都表现出对 ZB-15 的抗性，而对其他菌株的抗性则各不相同。值得注意的是，Pi-d2_H23 和 Pi-d2_H24 对所有测试的 M. oryzae 菌株都表现出抗性，表明这两个等位基因可能对 M. oryzae 具有更广泛的抗性谱。对这些等位基因的氨基酸序列进行比对后发现，抗瘟谱的差异主要与 PAN 结构域中位于 363 位（缬氨酸/丙氨酸）的氨基酸有关。这些发现表明，PI-D2 的两个胞外信号识别结构域（B-lectin 和 PAN）可能在识别 M. oryzae 效应子中发挥作用。本研究结果有助于深入了解 RLK 与 M. oryzae 之间的相互作用机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.