PRECISION AND RELIABILITY OF NANOPLATE DIGITAL PCR SYSTEM FOR PORK DNA IDENTIFICATION AND QUANTIFICATION

Abstract

The adulteration and mislabeling of food ingredients, particularly meat products, pose a significant concern for consumers. In order to address this issue, it is crucial to use precise and sensitive methods for species identification, mainly when dealing with samples that contain a limited number of copies of DNA. The primary objective of this study is to design a digital nanoplate polymerase chain reaction (PCR) technique for the purpose of quantifying species identification. The primer used in this study was cytochrome b gene. The results showed that target DNA can be amplified using the  digital PCR nanoplate until the lowest sample concentration of 0.0013 ng/µL. This study can be applied to enhance species identification processes in food samples by utilizing digital PCR nanoplates.