Molecular Detection of Antibiotics Profile of Citrobacter freundii Isolated from Burn Infection

Amaal M. Khudaier, Nawras M. Al-Khafaji, A. Al-Rubaeaee
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Abstract

Fifty samples were taken for this study from burn victims receiving care at the Al-Hussien medical city between February and October 2021. In order to isolate Citrobacter freundii, two swabs were taken: one for culturing and the other for direct examination. Only12 (4.1%) of the 50 samples were isolated from burn and wound infections at the molecular level and 12 (4.1%) from culture. The results isolation and laboratory diagnosis as well as biochemical test approved that there is only 12 isolates belong to C.freundii confirmed by molecular detection by specific primers. Burn and wound is one of the rare causes of infection. Associated with sub sequent urinary tract infection and burn and wound infection. Three antibiotics were employed at the molecular level , the results showed that 10 samples were resistant to the (Tet b)  gene, all samples were 100% sensitive to the PIP gene, and all samples (100% resistant to the erna gene).
烧伤感染中分离出的自由柠檬酸杆菌抗生素谱的分子检测
本研究从 2021 年 2 月至 10 月期间在胡先医疗城接受治疗的烧伤患者身上采集了 50 份样本。为了分离弗氏柠檬酸杆菌,采集了两个拭子:一个用于培养,另一个用于直接检查。在 50 份样本中,只有 12 份(4.1%)从烧伤和伤口感染中进行了分子水平分离,12 份(4.1%)从培养中进行了分离。分离和实验室诊断以及生化测试的结果表明,通过特异引物进行分子检测,只有 12 个分离物被确认为弗氏酵母菌。烧伤和伤口是罕见的感染原因之一。与尿路感染和烧伤及伤口感染有关。在分子水平上采用了三种抗生素,结果显示 10 个样本对(Tet b)基因耐药,所有样本对 PIP 基因 100%敏感,所有样本(对 erna 基因 100%耐药)。
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