Synthesis, MTT assay, 99m-Technetium radiolabeling, biodistribution evaluation of radiotracer and in vitro magnetic resonance imaging study of P,N-doped graphene quantum dots as a new multipurpose imaging nano-agent

IF 1.4 3区 化学 Q4 CHEMISTRY, INORGANIC & NUCLEAR
Morteza Mollazadeh, A. Fakhari, T. Mortezazadeh, F. B. Mofrad, Ali Jamali Nazarie
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Abstract

In this study, a new nano-structure, N,P-doped graphene quantum dots (N,P-GQDs), were synthesized as multipurpose imaging agent for performing scintigraphy and magnetic resonance imaging (MRI). Some standard characterization methods were used to identify the nano-structure. In vitro cytotoxicity evaluation using MTT assay revealed that N,P-GQDs nanoparticles had no significant cytotoxicity after 24 and 48 h against normal (MCF-10A) and cancerous (MCF 7) human breast cell line in concentration up to 200 μg/mL. The N,P-GQDs were radiolabeled with Technetium-99m as 99mTc-(N,P-GQDs) and the radiochemical purity was assayed by ITLC concluding RCP ≥ 95 %. The passing of 99mTc-(N,P-GQDs) through 0.1 µm filter demonstrated that 70.8 % of particles were <0.1 µm. In order to perform scintigraphy, the 99mTc-(N,P-GQDs) were injected to female healthy Wistar rats. The results showed that the radio-complex was captured and eliminated just by kidneys. Moreover, in vitro T1-weighted phantom MRI imaging showed that the N,P-GQDs have proper relaxivity in comparison to Dotarem® as a clinically available contrast agent. The results showed that the N,P-GQDs have potential to be considered as a novel and encouraging agent for both molecular MRI and nuclear medicine imagings.
作为新型多用途成像纳米试剂的 P,N 掺杂石墨烯量子点的合成、MTT 试验、99m-锝放射性标记、放射性示踪剂的生物分布评估和体外磁共振成像研究
本研究合成了一种新的纳米结构--N,P-掺杂石墨烯量子点(N,P-GQDs),它是一种多用途成像剂,可用于闪烁成像和磁共振成像(MRI)。研究人员采用了一些标准的表征方法来确定纳米结构。使用 MTT 法进行的体外细胞毒性评估显示,N,P-GQDs 纳米粒子在 24 和 48 小时后对正常(MCF-10A)和癌(MCF 7)人类乳腺细胞系没有明显的细胞毒性,浓度最高可达 200 μg/mL。N,P-GQDs经锝-99m放射性标记为99mTc-(N,P-GQDs),并通过ITLC测定其放射化学纯度,得出RCP≥95%的结论。99mTc-(N,P-GQDs) 通过 0.1 µm 过滤器的结果表明,70.8% 的颗粒小于 0.1 µm。为了进行闪烁成像,给健康的雌性 Wistar 大鼠注射了 99m锝-(N,P-GQDs)。结果表明,放射性复合物仅被肾脏捕获并清除。此外,体外 T1 加权模型核磁共振成像显示,与临床可用的造影剂 Dotarem® 相比,N,P-GQDs 具有适当的弛豫性。结果表明,N,P-GQDs 有潜力被视为分子磁共振成像和核医学成像中一种新型的、令人鼓舞的制剂。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Radiochimica Acta
Radiochimica Acta 化学-核科学技术
CiteScore
2.90
自引率
16.70%
发文量
78
审稿时长
6 months
期刊介绍: Radiochimica Acta publishes manuscripts encompassing chemical aspects of nuclear science and technology.
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