{"title":"Selective detection of biotinylated IL-6R protein using both CVD and epitaxial graphene-based electrochemical sensor and its reusability","authors":"Md. Zakir Hossain , Fumiya Nagasawa","doi":"10.1016/j.sbsr.2024.100659","DOIUrl":null,"url":null,"abstract":"<div><p>Here, we report the selective detection of biotinylated interleukin-6 receptor (IL-6R) protein using both chemical vapor deposition (CVD) and epitaxial graphene (EG) based electrochemical sensors and their reusability. Detection was based on the principle of avidin-biotin technology, which was widely used in different types of sensor technology. Following the characterization of graphene, the CVD and EG on SiC were fabricated with gold nanoparticles using our recently developed technique. The CVD graphene-based device was made by transferring the CVD graphene onto an interdigitated array electrode (IDA). In contrast, the EG-based device was made by photolithography by fabricating interdigital electrodes on EG on SiC. The detection of IL-6R was monitored by real-time two-terminal current measurements at fixed voltages, while the IL-6R protein was injected into avidin-immobilized graphene-based devices. Drops of the current (Ids) upon injection of as low as 50 pg/ml of IL-6R solution confirm the detection of IL-6R with ultra-high sensitivity. The specificity of the device was confirmed by a chicken egg white solution in PBS, which contains a variety of biomolecules. The EG on a SiC-based device can also be used to detect biomolecules with high sensitivity. The EG on the SiC-based device was found to be reusable after the physical cleaning procedure. The present study is expected to be exploited in the development of reusable ultra-sensitive point-of-care biosensors.</p></div>","PeriodicalId":424,"journal":{"name":"Sensing and Bio-Sensing Research","volume":null,"pages":null},"PeriodicalIF":5.4000,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214180424000412/pdfft?md5=2813b4cda808122cc752e191aec44935&pid=1-s2.0-S2214180424000412-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensing and Bio-Sensing Research","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214180424000412","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0
Abstract
Here, we report the selective detection of biotinylated interleukin-6 receptor (IL-6R) protein using both chemical vapor deposition (CVD) and epitaxial graphene (EG) based electrochemical sensors and their reusability. Detection was based on the principle of avidin-biotin technology, which was widely used in different types of sensor technology. Following the characterization of graphene, the CVD and EG on SiC were fabricated with gold nanoparticles using our recently developed technique. The CVD graphene-based device was made by transferring the CVD graphene onto an interdigitated array electrode (IDA). In contrast, the EG-based device was made by photolithography by fabricating interdigital electrodes on EG on SiC. The detection of IL-6R was monitored by real-time two-terminal current measurements at fixed voltages, while the IL-6R protein was injected into avidin-immobilized graphene-based devices. Drops of the current (Ids) upon injection of as low as 50 pg/ml of IL-6R solution confirm the detection of IL-6R with ultra-high sensitivity. The specificity of the device was confirmed by a chicken egg white solution in PBS, which contains a variety of biomolecules. The EG on a SiC-based device can also be used to detect biomolecules with high sensitivity. The EG on the SiC-based device was found to be reusable after the physical cleaning procedure. The present study is expected to be exploited in the development of reusable ultra-sensitive point-of-care biosensors.
期刊介绍:
Sensing and Bio-Sensing Research is an open access journal dedicated to the research, design, development, and application of bio-sensing and sensing technologies. The editors will accept research papers, reviews, field trials, and validation studies that are of significant relevance. These submissions should describe new concepts, enhance understanding of the field, or offer insights into the practical application, manufacturing, and commercialization of bio-sensing and sensing technologies.
The journal covers a wide range of topics, including sensing principles and mechanisms, new materials development for transducers and recognition components, fabrication technology, and various types of sensors such as optical, electrochemical, mass-sensitive, gas, biosensors, and more. It also includes environmental, process control, and biomedical applications, signal processing, chemometrics, optoelectronic, mechanical, thermal, and magnetic sensors, as well as interface electronics. Additionally, it covers sensor systems and applications, µTAS (Micro Total Analysis Systems), development of solid-state devices for transducing physical signals, and analytical devices incorporating biological materials.