{"title":"Comparative study of phytochemicals and pharmacological properties of different parts of Berberis sikkimensis (C. K. Schneid.) Ahrendt","authors":"Dheeraj Shootha , Deepika Tripathi , Mithilesh Singh , Purnima Rawat , Priyanka Srivastava , Devendra Kumar","doi":"10.1016/j.bse.2024.104850","DOIUrl":null,"url":null,"abstract":"<div><p>There is no scientific literature available on the chemical composition and biological activity of <em>Berberis</em> L. species of the Sikkim Himalayan region. The present investigation aimed to compare the phytochemical compositions and biological properties of leaf, fruit, and stem of <em>Berberis sikkimensis</em> (C.K.Schneid.) Ahrendt. Qualitative and quantitative methods were employed to analyze the phytochemicals present in the leaf, fruit, and stem extracts of <em>B. sikkimensis</em>. Biological activities such as antioxidant, antimicrobial, and DNA damage repair potential were evaluated <em>in vitro</em>. Qualitative screening of phytocompounds revealed the presence of phenol, flavonoids, tannin, saponin, alkaloids, and glycosides in the <em>B. sikkimensis</em> extracts. The contents of total phenol, flavonoids, and tannin were found to be significantly high in the methanolic leaf extract of <em>B. sikkimensis</em>. GC-MS analysis of the methanolic extracts detected the presence of 27 compounds in the leaf, 25 compounds in the fruit, and 29 compounds in the stem. Quinic acid was found to be the most abundant compound. Methanolic leaf extract exhibited the highest antioxidant activity. The antimicrobial results showed that the fruit extract has good antibacterial activity against gram-negative bacteria (<em>Escherichia coli</em> and <em>Serratia marcescens</em>). DNA damage repair potential was evaluated against oxidative stress, in which leaf methanolic extract showed maximum activity. Our results enlighten us that <em>B. sikkimensis</em> is an excellent source of bioactive compounds that can be used as a promising phytomedicine in the future.</p></div>","PeriodicalId":8799,"journal":{"name":"Biochemical Systematics and Ecology","volume":"115 ","pages":"Article 104850"},"PeriodicalIF":1.4000,"publicationDate":"2024-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemical Systematics and Ecology","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0305197824000681","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
There is no scientific literature available on the chemical composition and biological activity of Berberis L. species of the Sikkim Himalayan region. The present investigation aimed to compare the phytochemical compositions and biological properties of leaf, fruit, and stem of Berberis sikkimensis (C.K.Schneid.) Ahrendt. Qualitative and quantitative methods were employed to analyze the phytochemicals present in the leaf, fruit, and stem extracts of B. sikkimensis. Biological activities such as antioxidant, antimicrobial, and DNA damage repair potential were evaluated in vitro. Qualitative screening of phytocompounds revealed the presence of phenol, flavonoids, tannin, saponin, alkaloids, and glycosides in the B. sikkimensis extracts. The contents of total phenol, flavonoids, and tannin were found to be significantly high in the methanolic leaf extract of B. sikkimensis. GC-MS analysis of the methanolic extracts detected the presence of 27 compounds in the leaf, 25 compounds in the fruit, and 29 compounds in the stem. Quinic acid was found to be the most abundant compound. Methanolic leaf extract exhibited the highest antioxidant activity. The antimicrobial results showed that the fruit extract has good antibacterial activity against gram-negative bacteria (Escherichia coli and Serratia marcescens). DNA damage repair potential was evaluated against oxidative stress, in which leaf methanolic extract showed maximum activity. Our results enlighten us that B. sikkimensis is an excellent source of bioactive compounds that can be used as a promising phytomedicine in the future.
期刊介绍：
Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology).
In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.
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