Evaluation of coagulation and platelet activation state and function in heartworm-infected dogs

IF 1.2 4区农林科学 Q3 VETERINARY SCIENCES

Veterinary clinical pathology Pub Date : 2024-05-23 DOI:10.1111/vcp.13358

Carisa Fraser, Mandy L. Wallace, Andrew Moorhead, Jaime Tarigo, Benjamin M. Brainard

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引用次数: 0

Abstract

Background

Enhanced platelet responses have been demonstrated in heartworm-infected (HWI) dogs; however, the cause and clinical implications of altered platelet function have not been fully elucidated.

Objective

This study evaluated platelet function in HWI dogs.

Methods

Anticoagulated whole blood collected from eight HWI and eight uninfected dogs was evaluated using turbidometric platelet aggregometry, a platelet function analyzer (PFA-100), a total thrombus analysis system (T-TAS), tissue factor-activated and tissue plasminogen activator modified thromboelastography (TF- and tPA-TEG), CBC, von Willebrand Factor activity, and fibrinogen concentrations. Platelet activation state and the presence of reticulated platelets were assessed via flow cytometric expression of P-selection (CD-62P) and thiazole orange staining.

Results

Platelet aggregation responses to adenosine diphosphate (ADP, 10 μM) or collagen (20 μg/mL), PFA-100 closure times, and T-TAS occlusion times did not differ between groups. TEG values TF-R, tPA-R, TF-K, and TF-LY60 were decreased (P = .025, P = .047, P = .038, P = .025) and TF-MA, tPA-MA, TF-G, tPA-G and TF-alpha angle were increased (P < .04) in HWI dogs. HWI dogs had higher fibrinogen concentrations (465.6 ± 161 mg/dL vs 284.5 ± 38 mg/dL, P = .008) and eosinophil counts (0.686 ± 0.27 × 10³/μL vs 0.267 ± 0.20 × 10³/μL, P = .003). There was no difference in hematocrit, activation state, or percent of reticulated platelets. Non-activated reticulated platelets exhibited higher CD62P expression compared with mature platelets.

Conclusions

Chronic canine heartworm disease was accompanied by hypercoagulability, hyperfibrinogenemia, and decreased fibrinolysis. Enhanced platelet activation was not identified in this group of HWI dogs.

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评估心丝虫感染犬的凝血和血小板活化状态及功能。

背景：已证实心丝虫感染（HWI）犬的血小板反应增强；然而，血小板功能改变的原因和临床意义尚未完全阐明：本研究评估了 HWI 犬的血小板功能：方法：使用浊度血小板聚集测定法、血小板功能分析仪（PFA-100）、全血栓分析系统（T-TAS）、组织因子活化和组织纤溶酶原激活剂修饰血栓弹力图（TF- 和 tPA-TEG）、全血细胞计数、冯-威廉因子活性和纤维蛋白原浓度对从 8 只 HWI 犬和 8 只未感染犬采集的抗凝全血进行评估。血小板活化状态和网状血小板的存在通过流式细胞术表达 P 选择（CD-62P）和噻唑橙染色进行评估：结果：血小板对二磷酸腺苷（ADP，10 μM）或胶原蛋白（20 μg/mL）的聚集反应、PFA-100闭合时间和T-TAS闭合时间在组间无差异。TEG值TF-R、tPA-R、TF-K和TF-LY60降低（P = .025、P = .047、P = .038、P = .025），TF-MA、tPA-MA、TF-G、tPA-G和TF-α角升高（P 3/μL vs 0.267 ± 0.20 × 103/μL，P = .003）。血细胞比容、活化状态或网状血小板百分比没有差异。与成熟血小板相比，未活化的网状血小板的 CD62P 表达更高：慢性犬心丝虫病伴有高凝状态、高纤维蛋白原血症和纤维蛋白溶解减少。在这组 HWI 犬中未发现血小板活化增强。

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来源期刊

Veterinary clinical pathology 农林科学-兽医学

CiteScore

1.70

自引率

16.70%

发文量

133

审稿时长

18-36 weeks

期刊介绍： Veterinary Clinical Pathology is the official journal of the American Society for Veterinary Clinical Pathology (ASVCP) and the European Society of Veterinary Clinical Pathology (ESVCP). The journal''s mission is to provide an international forum for communication and discussion of scientific investigations and new developments that advance the art and science of laboratory diagnosis in animals. Veterinary Clinical Pathology welcomes original experimental research and clinical contributions involving domestic, laboratory, avian, and wildlife species in the areas of hematology, hemostasis, immunopathology, clinical chemistry, cytopathology, surgical pathology, toxicology, endocrinology, laboratory and analytical techniques, instrumentation, quality assurance, and clinical pathology education.