Effect of oral administration of microcin Y on growth performance, intestinal barrier function and gut microbiota of chicks challenged with Salmonella Pullorum.

IF 3.7 1区 农林科学 Q1 VETERINARY SCIENCES
Wenjing Li, Zhiwei Zeng, Di Zhou, Guyao Wang, Zepeng Wang, Yu Li, Yu Han, Miaomiao Qin, Changqi Luo, Saixiang Feng, Weisheng Cao
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引用次数: 0

Abstract

The lasso peptide microcin Y (MccY) effectively inhibits various serotypes of Salmonella in vitro, but the antibacterial effect against S. Pullorum in poultry is still unclear. This study was the first to evaluate the safety and anti-S. Pullorum infection of MccY in specific pathogen-free (SPF) chicks. The safety test showed that the body weight, IgA and IgM levels of serum, and cecal microbiota structure of 3 groups of chicks orally administrated with different doses of MccY (5 mg/kg, 10 mg/kg, 20 mg/kg) for 14 days were not significantly different from those of the control group. Then, the chicks were randomized into 3 groups for the experiment of anti-S. Pullorum infection: (I) negative control group (NC), (II) S. Pullorum-challenged group (SP, 5 × 108 CFU/bird), (III) MccY-treated group (MccY, 20 mg/kg). The results indicated that compared to the SP group, treatment of MccY increased body weight and average daily gain (P < 0.05), reduced S. Pullorum burden in feces, liver, and cecum (P < 0.05), enhanced the thymus, and decreased the spleen and liver index (P < 0.05). Additionally, MccY increased the jejunal villus height, lowered the jejunal and ileal crypt depth (P < 0.05), and upregulated the expression of IL-4, IL-10, ZO-1 in the jejunum and ileum, as well as CLDN-1 in the jejunum (P < 0.05) compared to the SP group. Furthermore, MccY increased probiotic flora (Barnesiella, etc.), while decreasing (P < 0.05) the relative abundance of pathogenic flora (Escherichia and Salmonella, etc.) compared to the SP group.

口服微量霉素 Y 对雏鸡生长性能、肠道屏障功能和普氏沙门氏菌肠道微生物群的影响
拉索肽 microcin Y(MccY)在体外能有效抑制多种血清型的沙门氏菌,但对家禽中普氏沙门氏菌的抗菌效果仍不清楚。本研究首次评估了 MccY 在家禽中的安全性和抗沙门氏菌感染的效果。本研究首次评估了 MccY 在无特定病原体(SPF)雏鸡中的安全性和抗 S. Pullorum 感染性。安全性试验表明,口服不同剂量的MccY(5毫克/千克、10毫克/千克、20毫克/千克)14天后,3组雏鸡的体重、血清中的IgA和IgM水平以及盲肠微生物群结构与对照组无显著差异。然后,将雏鸡随机分为 3 组进行抗 S. Pullorum 感染实验:(I)阴性组;(II)阳性组;(III)阴性组。(I)阴性对照组(NC),(II)普氏原虫挑战组(SP,5 × 108 CFU/只),(III)MccY处理组(MccY,20 mg/kg)。结果表明,与 SP 组相比,MccY 治疗组的体重和平均日增重均有所增加(P
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来源期刊
Veterinary Research
Veterinary Research 农林科学-兽医学
CiteScore
7.00
自引率
4.50%
发文量
92
审稿时长
3 months
期刊介绍: Veterinary Research is an open access journal that publishes high quality and novel research and review articles focusing on all aspects of infectious diseases and host-pathogen interaction in animals.
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