LncRNA AC100826.1 regulated PLCB1 to promote progression in non-small cell lung cancer.

IF 2.3 3区医学 Q3 ONCOLOGY

Thoracic Cancer Pub Date : 2024-07-01 Epub Date: 2024-05-22 DOI:10.1111/1759-7714.15323

Shenhui Dai, Qiao Wang, Yin Lyu, Zhipeng Chen, Xiucheng Liu, Guoqing Zhao, Hao Zhang

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引用次数: 0

Abstract

Background: Lung cancer is the most common malignant tumor. In the present study, we identified a long non-coding RNA (lncRNA) AC100826.1 (simplify to Lnc1), which was highly expressed in non-small cell lung cancer (NSCLC) tissues compared with the paracancerous tissues. We also observed the critical role of Lnc1 in regulating the metastasis ability of NSCLC cells.

Methods: RNA sequencing was performed to detect differential expression levels of lncRNAs in NSCLC tissues and its paracancerous tissues. Effects of Lnc1 on cell proliferation, invasion, and migration were determined by CCK-8, transwell and scratch assays. The xenograft experiment confirmed the effect of Lnc1 on NSCLC cells proliferation and migration abilities in vivo. RT-qPCR and western blots were performed to determine the expression levels of mRNAs and proteins.

Results: The expression level of Lnc1 was related to multiple pathological results, knockdown of Lnc1 can inhibit the proliferation and metastasis abilities of NSCLC cells. silencing phospholipase C, β1(PLCB1) can reverse the promoting effects of overexpression Lnc1 on NSCLC cells proliferation and migration abilities. In addition, the Rap1 signaling pathway was implicated in the regulation of Lnc1 in NSCLC metastasis.

Conclusion: Our results suggest that Lnc1 regulated the metastatic ability of NSCLC cells through targeting the PLCB1/Rap1 signal pathway.

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LncRNA AC100826.1调控PLCB1以促进非小细胞肺癌的进展。

背景：肺癌是最常见的恶性肿瘤：肺癌是最常见的恶性肿瘤。在本研究中，我们发现了一种长非编码 RNA（lncRNA）AC100826.1（简化为 Lnc1），与癌旁组织相比，它在非小细胞肺癌（NSCLC）组织中高表达。我们还观察到 Lnc1 在调控 NSCLC 细胞转移能力中的关键作用：方法：通过RNA测序检测NSCLC组织及其癌旁组织中lncRNAs的不同表达水平。Lnc1对细胞增殖、侵袭和迁移的影响通过CCK-8、跨孔实验和划痕实验进行测定。异种移植实验证实了 Lnc1 对 NSCLC 细胞增殖和迁移能力的影响。RT-qPCR 和 Western 印迹检测了 mRNA 和蛋白质的表达水平：结果：Lnc1的表达水平与多种病理结果有关，敲除Lnc1可抑制NSCLC细胞的增殖和转移能力；沉默磷脂酶C，β1（PLCB1）可逆转过表达Lnc1对NSCLC细胞增殖和迁移能力的促进作用。此外，Rap1信号通路也参与了Lnc1在NSCLC转移中的调控：我们的研究结果表明，Lnc1通过靶向PLCB1/Rap1信号通路调控NSCLC细胞的转移能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Thoracic Cancer ONCOLOGY-RESPIRATORY SYSTEM

CiteScore

5.20

自引率

3.40%

发文量

439

审稿时长

2 months

期刊介绍： Thoracic Cancer aims to facilitate international collaboration and exchange of comprehensive and cutting-edge information on basic, translational, and applied clinical research in lung cancer, esophageal cancer, mediastinal cancer, breast cancer and other thoracic malignancies. Prevention, treatment and research relevant to Asia-Pacific is a focus area, but submissions from all regions are welcomed. The editors encourage contributions relevant to prevention, general thoracic surgery, medical oncology, radiology, radiation medicine, pathology, basic cancer research, as well as epidemiological and translational studies in thoracic cancer. Thoracic Cancer is the official publication of the Chinese Society of Lung Cancer, International Chinese Society of Thoracic Surgery and is endorsed by the Korean Association for the Study of Lung Cancer and the Hong Kong Cancer Therapy Society. The Journal publishes a range of article types including: Editorials, Invited Reviews, Mini Reviews, Original Articles, Clinical Guidelines, Technological Notes, Imaging in thoracic cancer, Meeting Reports, Case Reports, Letters to the Editor, Commentaries, and Brief Reports.