Bilateral bronchoalveolar lavage cytology profiles in a warmblood horse population during a 1-year period

IF 2.1 2区农林科学 Q1 VETERINARY SCIENCES

Journal of Veterinary Internal Medicine Pub Date : 2024-05-23 DOI:10.1111/jvim.17118

Nanna Rasmussen, Pernille Karlsen, Nina D. Otten, Julie Fjeldborg, Sanni Hansen

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引用次数: 0

Abstract

Background

Bronchoalveolar lavage (BAL) cytology results from 1 lung might not be representative of both lungs.

Objectives

To determine whether the lung site sampled would influence the horse's BAL cytology profile, and if a pooled BAL sample would be superior with regard to BAL cytology diagnosis in a cohort of healthy and subclinical asthmatic warmblood horses.

Animals

Fifty-nine horses in 2021 and 70 horses in 2022, the follow-up included 53 of the same in each year.

Methods

A cross-sectional study with follow-up included BAL cytology samples from individual lungs and from pooled BAL samples. The BAL samples were enumerated and differential cell count were applied to categorize the horses as control or with airway inflammation (AI).

Results

Bronchoalveolar lavage mast cell count was higher in left lung compared to right lung (2021; median 1.6 [range, 0.6-3.3] vs 1.2 [0.7-1.5] P = .009, 2022; median 3.1 [2.1-4.2] vs 2.4 [1.7-3.4], P < .001) and compared to pooled samples (2022; median 2.6 [1.7-3.7], P < .001). Between year 2021 and 2022, 17 of the horses had changes in BAL cytology from control to AI or vice versa.

Conclusions and Clinical Importance

Pooled BAL sample was the least reliable for detecting AI, and was not representative of the overall lung condition.

查看原文本刊更多论文

在 1 年时间里，温血马群体的双侧支气管肺泡灌洗细胞学概况。

背景：一个肺的支气管肺泡灌洗（BAL）细胞学结果可能不能代表两个肺：来自一个肺部的支气管肺泡灌洗（BAL）细胞学结果可能无法代表两个肺部的情况：确定取样的肺部部位是否会影响马匹的 BAL 细胞学特征，以及在健康和亚临床哮喘温血马队列中，汇总 BAL 样本是否更有利于 BAL 细胞学诊断：动物：2021年59匹马，2022年70匹马，每年随访53匹相同的马：随访横断面研究包括来自单个肺部的 BAL 细胞学样本和来自汇集的 BAL 样本。对 BAL 样本进行计数，并采用细胞计数差法将马匹分为对照组和气道炎症（AI）组：结果：与右肺相比，左肺支气管肺泡灌洗肥大细胞计数更高（2021 年；中位数 1.6 [范围，0.6-3.3] vs 1.2 [0.7-1.5] P = .009，2022 年；中位数 3.1 [2.1-4.2] vs 2.4 [1.7-3.4]，P 结论和临床重要性：汇总的 BAL 样本在检测 AI 方面最不可靠，也不能代表整体肺部状况。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Veterinary Internal Medicine 农林科学-兽医学

CiteScore

4.50

自引率

11.50%

发文量

243

审稿时长

22 weeks

期刊介绍： The mission of the Journal of Veterinary Internal Medicine is to advance veterinary medical knowledge and improve the lives of animals by publication of authoritative scientific articles of animal diseases.