Genome-wide identification of walnut (Juglans regia) PME gene family members and expression analysis during infection with Cryptosphaeria pullmanensis pathogens

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-05-20 DOI:10.1016/j.ygeno.2024.110860

Ze Qin , Chengcai Yan , Kaiying Yang, Qinpeng Wang, Zhe Wang, Changqing Gou, Hongzu Feng, Qiming Jin, Xianxing Dai, Zulihumar Maitikadir, Haiting Hao, Lan Wang

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引用次数: 0

Abstract

Walnuts exhibit a higher resistance to diseases, though they are not completely immune. This study focuses on the Pectin methylesterase (PME) gene family to investigate whether it is involved in disease resistance in walnuts. These 21 genes are distributed across 12 chromosomes, with four pairs demonstrating homology. Variations in conserved motifs and gene structures suggest diverse functions within the gene family. Phylogenetic and collinear gene pairs of the PME family indicate that the gene family has evolved in a relatively stable way. The cis-acting elements and gene ontology enrichment of these genes, underscores their potential role in bolstering walnuts' defense mechanisms. Transcriptomic analyses were conducted under conditions of Cryptosphaeria pullmanensis infestation and verified by RT-qPCR. The results showed that certain JrPME family genes were activated in response, leading to the hypothesis that some members may confer resistance to the disease.

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核桃（Juglans regia）PME 基因家族成员的全基因组鉴定及感染拉曼隐杆线虫病原体期间的表达分析。

核桃具有较强的抗病能力，但并非完全免疫。这项研究的重点是果胶甲基酯酶（PME）基因家族，研究它是否与核桃的抗病性有关。这 21 个基因分布在 12 条染色体上，其中 4 对基因具有同源性。保守基序和基因结构的变化表明该基因家族具有多种功能。PME 基因家族的系统发育和基因对的共线性表明，该基因家族是以相对稳定的方式进化的。这些基因的顺式作用元件和基因本体论富集强调了它们在增强核桃防御机制中的潜在作用。在拉曼隐杆线虫侵袭条件下进行了转录组分析，并通过 RT-qPCR 进行了验证。结果表明，JrPME 家族的某些基因被激活，从而提出了某些成员可能具有抗病能力的假设。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567