The mechanism of action of Botrychium (Thunb.) Sw. for prevention of idiopathic pulmonary fibrosis based on 1H-NMR-based metabolomics.

IF 2.8 4区医学 Q2 PHARMACOLOGY & PHARMACY

Journal of Pharmacy and Pharmacology Pub Date : 2024-08-02 DOI:10.1093/jpp/rgae058

Yutao Lou, Xiaozhou Zou, Zongfu Pan, Zhongjie Huang, Shuilian Zheng, Xiaowei Zheng, Xiuli Yang, Meihua Bao, Yuan Zhang, Jinping Gu, Yiwen Zhang

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引用次数: 0

Abstract

Objectives: This study aimed to reveal the anti-fibrotic effects of Botrychium ternatum (Thunb.) Sw. (BT) against idiopathic pulmonary fibrosis (IPF) and to preliminarily analyze its potential mechanism on bleomycin-induced IPF rats.

Methods: The inhibition of fibrosis progression in vivo was assessed by histopathology combined with biochemical indicators. In addition, the metabolic regulatory mechanism was investigated using 1H-nuclear magnetic resonance-based metabolomics combined with multivariate statistical analysis.

Key findings: Firstly, biochemical analysis revealed that BT notably suppressed the expression of hydroxyproline and transforming growth factor-β1 in the pulmonary tissue. Secondly, Masson's trichrome staining and hematoxylin and eosin showed that BT substantially improved the structure of the damaged lung and significantly inhibited the proliferation of collagen fibers and the deposition of extracellular matrix. Finally, serum metabolomic analysis suggested that BT may exert anti-fibrotic effects by synergistically regulating tyrosine metabolism; phenylalanine, tyrosine and tryptophan biosynthesis; and synthesis and degradation of ketone bodies.

Conclusions: Our study not only clarifies the potential anti-fibrotic mechanism of BT against IPF at the metabolic level but also provides a theoretical basis for developing BT as an effective anti-fibrotic agent.

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基于 1H-NMR 代谢组学的 Botrychium (Thunb.) Sw. 预防特发性肺纤维化的作用机制。

研究目的本研究旨在揭示 Botrychium ternatum (Thunb.) Sw.(BT）对特发性肺纤维化（IPF）的抗纤维化作用，并初步分析其对博莱霉素诱导的IPF大鼠的潜在机制：方法：通过组织病理学和生化指标评估 BT 对体内纤维化进展的抑制作用。此外，还利用基于 1H 核磁共振的代谢组学结合多元统计分析研究了代谢调控机制：首先，生化分析表明，BT 显著抑制了肺组织中羟脯氨酸和转化生长因子-β1 的表达。其次，Masson 三色染色和苏木精及伊红显示，BT 能显著改善受损肺部的结构，明显抑制胶原纤维的增殖和细胞外基质的沉积。最后，血清代谢组学分析表明，BT 可通过协同调节酪氨酸代谢、苯丙氨酸、酪氨酸和色氨酸的生物合成以及酮体的合成和降解来发挥抗纤维化作用：我们的研究不仅从代谢层面阐明了 BT 抗 IPF 的潜在抗纤维化机制，还为将 BT 开发为有效的抗纤维化药物提供了理论依据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Pharmacy and Pharmacology 医学-药学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

3 months

期刊介绍： JPP keeps pace with new research on how drug action may be optimized by new technologies, and attention is given to understanding and improving drug interactions in the body. At the same time, the journal maintains its established and well-respected core strengths in areas such as pharmaceutics and drug delivery, experimental and clinical pharmacology, biopharmaceutics and drug disposition, and drugs from natural sources. JPP publishes at least one special issue on a topical theme each year.

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