Investigation of reno-protective efficacy of thymoquinone in a unilateral hydronephrosis model.

IF 1.6 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Erdem Özatman, Burhan Aksu, Itir Ebru Zemheri, Hayriye Erman, Çiğdem Ulukaya Durakbaşa
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引用次数: 0

Abstract

We aimed to evaluate the effects of the antioxidant thymoquinone on treated and untreated kidneys on histological and oxidative parameters as well as Kidney Injury Molecule (KIM-1) levels in an experimental unilateral ureteropelvic junction obstruction (UPJO) with resultant hydronephrosis (HN) model. In adherence to the Animal research: reporting of in vivo exepriments guidelines, 34 male Wistar rats were randomly divided into four groups which were named accordingly: "CO" (corn oil), "TQ" (thymoquinone and corn oil), "HNCO" (UPJO-HN and corn oil), "HNTQ" (UPJO-HN, thymoquinone and corn oil). Histologically, pelvic epithelial damage, glomerular shrinkage and sclerosis, tubular damage, interstitial edema-inflammation-fibrosis (IEIF), and vascular congestion were assessed. Biochemically, malondialdehyde (MDA), superoxide dismutase (SOD), glutathione reductase (GR) and KIM-1 levels were assessed. Macroscopic HN developed in all obstructed kidneys. Ipsilateral obstructed kidneys deteriorated in all histological parameters. Thymoquinone attenuated glomerular shrinkage and sclerosis alterations but increased vascular congestion. Contralateral non-obstructed kidneys also showed histological deterioration. Thymoquinone had beneficial effects in terms of IEIF presence in contralateral kidneys but it increased vascular congestion. MDA and SOD results were inconclusive. UPJO caused decreased GR levels in the ipsilateral kidneys but not in the contralateral ones. This effect was not ameliorated by thymoquinone treatment. KIM-1 levels were increased in ipsilateral obstructed kidneys with a lower level in HNTQ group than in HNCO. KIM-1 level of the ipsilateral HNTQ group was higher than in both non-obstructed ipsilateral kidney groups. The effect of thymoquinone in ameliorating bilaterally observed histological alterations was limited and controversial. Oxidative damage detected by GR measurements was not prevented by thymoquinone. Thymoquinone partially decreased the damage as evidenced by reduced KIM-1 levels in thymoquinone-treated obstructed kidneys.

在单侧肾积水模型中研究胸腺醌的肾脏保护功效
我们的目的是评估抗氧化剂胸腺醌在实验性单侧输尿管肾盂连接处梗阻(UPJO)并导致肾积水(HN)模型中,对治疗和未治疗肾脏的组织学和氧化参数以及肾损伤分子(KIM-1)水平的影响。根据《动物研究:活体实验报告指南》,34 只雄性 Wistar 大鼠被随机分为四组,并分别命名为 "CO "组(玉米油)、"KIM-1 "组(KIM-1)和 "CO "组(玉米油):"CO"(玉米油)、"TQ"(胸腺醌和玉米油)、"HNCO"(UPJO-HN 和玉米油)、"HNTQ"(UPJO-HN、胸腺醌和玉米油)。在组织学方面,对盆腔上皮损伤、肾小球萎缩和硬化、肾小管损伤、间质水肿-炎症-纤维化(IEIF)和血管充血进行了评估。生化指标包括丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽还原酶(GR)和 KIM-1 水平。所有梗阻肾脏均出现宏观HN。同侧梗阻肾脏的所有组织学参数均恶化。胸腺醌减轻了肾小球萎缩和硬化改变,但增加了血管充血。对侧非梗阻性肾脏也出现了组织学恶化。胸腺醌对对侧肾脏中 IEIF 的存在有好处,但会加重血管充血。MDA 和 SOD 的结果尚无定论。UPJO 导致同侧肾脏的 GR 水平下降,但对侧肾脏的 GR 水平没有下降。胸腺醌治疗并不能改善这种影响。同侧梗阻肾脏的 KIM-1 水平升高,而 HNTQ 组的 KIM-1 水平低于 HNCO 组。同侧 HNTQ 组的 KIM-1 水平高于非梗阻同侧肾脏组。胸腺醌对改善双侧观察到的组织学改变的作用有限,且存在争议。胸腺醌不能阻止通过GR测量发现的氧化损伤。胸腺醌可部分减轻损伤,胸腺醌处理的梗阻肾脏中KIM-1水平的降低就证明了这一点。
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来源期刊
Biotechnic & Histochemistry
Biotechnic & Histochemistry 生物-生物工程与应用微生物
CiteScore
3.40
自引率
6.20%
发文量
46
审稿时长
6-12 weeks
期刊介绍: Biotechnic & Histochemistry (formerly Stain technology) is the official publication of the Biological Stain Commission. The journal has been in continuous publication since 1926. Biotechnic & Histochemistry is an interdisciplinary journal that embraces all aspects of techniques for visualizing biological processes and entities in cells, tissues and organisms; papers that describe experimental work that employs such investigative methods are appropriate for publication as well. Papers concerning topics as diverse as applications of histochemistry, immunohistochemistry, in situ hybridization, cytochemical probes, autoradiography, light and electron microscopy, tissue culture, in vivo and in vitro studies, image analysis, cytogenetics, automation or computerization of investigative procedures and other investigative approaches are appropriate for publication regardless of their length. Letters to the Editor and review articles concerning topics of special and current interest also are welcome.
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