Morphological and molecular characterization of parabasilids isolated from ex situ nonhuman primates and their keepers at different institutions in Brazil
Lais Verdan Dib , Alynne da Silva Barbosa , Lais Lisboa Correa , Breno da Silva Torres , Alcides Pissinatti , Silvia Bahadian Moreira , Rodrigo Hidalgo Friciello Teixeira , André Luíz Mota da Costa , José Augusto Pereira Carneiro Muniz , Amauri Michel Junglos , Zelinda Maria Braga Hirano , Maria Regina Reis Amendoeira
{"title":"Morphological and molecular characterization of parabasilids isolated from ex situ nonhuman primates and their keepers at different institutions in Brazil","authors":"Lais Verdan Dib , Alynne da Silva Barbosa , Lais Lisboa Correa , Breno da Silva Torres , Alcides Pissinatti , Silvia Bahadian Moreira , Rodrigo Hidalgo Friciello Teixeira , André Luíz Mota da Costa , José Augusto Pereira Carneiro Muniz , Amauri Michel Junglos , Zelinda Maria Braga Hirano , Maria Regina Reis Amendoeira","doi":"10.1016/j.ijppaw.2024.100946","DOIUrl":null,"url":null,"abstract":"<div><p>Intestinal protozoa, which can be asymptomatic or cause diarrhea, dysentery and even death, are among the main agents that affect nonhuman primates (NHPs) kept under human care. Nevertheless, information on the molecular and morphometric profiles of parabasilids in the Neotropics is still scarce. In this context, the objective of this study was to isolate the Parabasalia protozoa detected in the feces of NHPs and their keepers in Pavlova and TYSGM9 media and to characterize the isolates by molecular biology and morphometry. Fecal samples from NHPs from five Brazilian institutions were analyzed. Direct examination was performed immediately after obtaining the samples. A total of 511 fecal samples from NHPs were collected, and 10.6% contained parabasilids. Regarding the handlers, of the 74 samples analyzed, three were positive. In vitro-generated parabasilid isolates were successfully obtained from all positive samples, as identified via microscopy. Isolates of the parasite were obtained both from New World NHPs, including the genera <em>Leontopithecus</em>, <em>Saguinus</em>, <em>Leontocebus</em>, <em>Aotus</em>, <em>Saimiri</em>, <em>Sapajus</em>, and <em>Alouatta,</em> and from the Old World primate <em>Pan troglodytes</em>. Forty-nine NHP isolates were molecularly identified: <em>Pentatrichomonas hominis</em> (16), <em>Trichomitus batrachorum</em> (14), <em>Tetratrichomonas brumpti</em> (13) and <em>Hypotrichomonas hampli</em> (6). The human isolates were identified as <em>Tetratrichomonas</em> sp. (2) and <em>T. batrachorum</em> (1). Visualization and morphometric analysis revealed trophozoites with piriform or rounded shapes that presented variable measurements. The isolates previously characterized as <em>P. hominis</em> had up to five free flagella, while <em>T. batrachorum</em> and <em>Tetratrichomonas</em> sp. had up to four free flagella, and <em>H. hampli</em> had a maximum of three free flagella. These morphometric characteristics corroborated the molecular identification. In general, a variety of parabasilids were observed to infect NHPs, and <em>T. batrachorum</em> was isolated from biological samples from both NHPs and their keepers, a finding that reinforces the susceptibility of these hosts to infections by parabasilids in Brazil.</p></div>","PeriodicalId":54278,"journal":{"name":"International Journal for Parasitology-Parasites and Wildlife","volume":"24 ","pages":"Article 100946"},"PeriodicalIF":2.0000,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2213224424000427/pdfft?md5=9f24d5cb8b2e34c87b7eff27f1ecab6c&pid=1-s2.0-S2213224424000427-main.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal for Parasitology-Parasites and Wildlife","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2213224424000427","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"ECOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Intestinal protozoa, which can be asymptomatic or cause diarrhea, dysentery and even death, are among the main agents that affect nonhuman primates (NHPs) kept under human care. Nevertheless, information on the molecular and morphometric profiles of parabasilids in the Neotropics is still scarce. In this context, the objective of this study was to isolate the Parabasalia protozoa detected in the feces of NHPs and their keepers in Pavlova and TYSGM9 media and to characterize the isolates by molecular biology and morphometry. Fecal samples from NHPs from five Brazilian institutions were analyzed. Direct examination was performed immediately after obtaining the samples. A total of 511 fecal samples from NHPs were collected, and 10.6% contained parabasilids. Regarding the handlers, of the 74 samples analyzed, three were positive. In vitro-generated parabasilid isolates were successfully obtained from all positive samples, as identified via microscopy. Isolates of the parasite were obtained both from New World NHPs, including the genera Leontopithecus, Saguinus, Leontocebus, Aotus, Saimiri, Sapajus, and Alouatta, and from the Old World primate Pan troglodytes. Forty-nine NHP isolates were molecularly identified: Pentatrichomonas hominis (16), Trichomitus batrachorum (14), Tetratrichomonas brumpti (13) and Hypotrichomonas hampli (6). The human isolates were identified as Tetratrichomonas sp. (2) and T. batrachorum (1). Visualization and morphometric analysis revealed trophozoites with piriform or rounded shapes that presented variable measurements. The isolates previously characterized as P. hominis had up to five free flagella, while T. batrachorum and Tetratrichomonas sp. had up to four free flagella, and H. hampli had a maximum of three free flagella. These morphometric characteristics corroborated the molecular identification. In general, a variety of parabasilids were observed to infect NHPs, and T. batrachorum was isolated from biological samples from both NHPs and their keepers, a finding that reinforces the susceptibility of these hosts to infections by parabasilids in Brazil.
期刊介绍：
The International Journal for Parasitology: Parasites and Wildlife (IJP-PAW) publishes the results of original research on parasites of all wildlife, invertebrate and vertebrate. This includes free-ranging, wild populations, as well as captive wildlife, semi-domesticated species (e.g. reindeer) and farmed populations of recently domesticated or wild-captured species (e.g. cultured fishes). Articles on all aspects of wildlife parasitology are welcomed including taxonomy, biodiversity and distribution, ecology and epidemiology, population biology and host-parasite relationships. The impact of parasites on the health and conservation of wildlife is seen as an important area covered by the journal especially the potential role of environmental factors, for example climate. Also important to the journal is ''one health'' and the nature of interactions between wildlife, people and domestic animals, including disease emergence and zoonoses.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
