Cytotoxic activities of metallic nanoparticles biosynthesized using plant extracts and pure compounds from Cephalaria species

IF 1.3 4区 生物学 Q4 CHEMISTRY, MEDICINAL
Mohamed Chanfiou Mkouboi , Nazli Boke Sarikahya , Ayse Nalbantsoy , Murat Elibol
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引用次数: 0

Abstract

Green nanotechnology has been focused on new concepts and fundamental research to produce nanoparticles in high rates and low risks by the use of different sources such as microorganisms and plants. Due to the several constituents in secondary metabolites, plants play an important role in nanomaterial synthesis known as ecofriendly methods which acquired important potential in different studies. The current work aimed to synthesize different silver and zinc-based nanoparticles using Cephalaria tchihatchewii via phytobiological methods involving metallic solutions, organic extracts, and isolated compounds. Sixteen types of nanoparticles were determined in between 50 and 297 nm as diameter and characterized using ultraviolet-visible spectrophotometer, dynamic light scattering, scanning electron microscopy and fourier transform infrared spectroscopy. The cytotoxic activities of nanoparticles were evaluated against human lung cancer (A549), breast cancer (MDA-MB-231), prostate cancer (PC3), and healthy lung (CCD-34Lu) cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2 H-tetrazolium bromide MTT assay utilizing doxorubicin as a positive control. Against PC3 cells, the cytotoxic activity of silver nitrate and loganin-AgNPs show significant inhibition with IC50 values of 2.39 ± 0.16 and 7.84 ± 1.21 respectively compared with doxorubicin (12.99 ± 1.57 µg/mL). A moderate inhibition activity was observed for Zinc sulfate, elmalienoside-B-AgNPs, methyl glycoside-AgNPs, and luteolin-7-O-glycoside-AgNPs with IC50 values of 22. 27± 0.18, 11.59 ± 0.82, 12.43 ± 0.37 and 13.26 ± 1.63 µg/mL, respectively. The inhibition activity against human breast cancer cells MDA-MB-231 was significantly reported with silver nitrate (2.25 ± 0.62 µg/mL), and elmalienoside-B-AgNPs (11.48 ± 0.46 µg/mL) compared with doxorubicin (11.61± 0.68 µg/mL). The cytotoxicity activities, make Cephalaria tchihatchewii plant a suitable candidate in different biopharmaceutical usages especially in anticancer activities. This study will provide a clear perspective on nanoparticles containing green synthesized pure compounds and the reaction mechanism of their activities.

Abstract Image

利用头孢属植物提取物和纯化合物生物合成的金属纳米粒子的细胞毒活性
绿色纳米技术一直专注于利用微生物和植物等不同来源生产纳米粒子的新概念和基础研究。由于次生代谢物中含有多种成分,植物在纳米材料合成中发挥着重要作用,被称为生态友好型方法,在不同的研究中获得了重要的潜力。目前的工作旨在通过植物生物学方法(包括金属溶液、有机提取物和分离化合物),利用头孢蘑菇(Cephalaria tchihatchewii)合成不同的银基和锌基纳米粒子。通过紫外可见分光光度计、动态光散射、扫描电子显微镜和傅立叶变换红外光谱测定了 16 种直径在 50 至 297 nm 之间的纳米粒子,并对其进行了表征。以多柔比星为阳性对照,采用 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基-2 H-溴化四氮唑 MTT 法评估了纳米颗粒对人类肺癌(A549)、乳腺癌(MDA-MB-231)、前列腺癌(PC3)和健康肺(CCD-34Lu)细胞系的细胞毒活性。与多柔比星(12.99 ± 1.57 µg/mL)相比,硝酸银和 loganin-AgNPs 对 PC3 细胞的细胞毒性活性具有显著的抑制作用,IC50 值分别为 2.39 ± 0.16 和 7.84 ± 1.21。硫酸锌、地榆苷-B-AgNPs、甲基糖苷-AgNPs 和叶黄素-7-O-糖苷-AgNPs 具有中等抑制活性,IC50 值分别为 22.27±0.18、11.59±0.82、12.43±0.37 和 13.26±1.63 µg/mL。与多柔比星(11.61± 0.68 µg/mL)相比,硝酸银(2.25± 0.62 µg/mL)和地榆烯苷-B-AgNPs(11.48± 0.46 µg/mL)对人乳腺癌细胞 MDA-MB-231 的抑制活性显著。这些细胞毒性活性使头状花序植物成为不同生物制药用途的合适候选者,尤其是在抗癌活性方面。这项研究将为含有绿色合成纯化合物的纳米颗粒及其活性反应机制提供一个清晰的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Phytochemistry Letters
Phytochemistry Letters 生物-生化与分子生物学
CiteScore
3.00
自引率
11.80%
发文量
190
审稿时长
34 days
期刊介绍: Phytochemistry Letters invites rapid communications on all aspects of natural product research including: • Structural elucidation of natural products • Analytical evaluation of herbal medicines • Clinical efficacy, safety and pharmacovigilance of herbal medicines • Natural product biosynthesis • Natural product synthesis and chemical modification • Natural product metabolism • Chemical ecology • Biotechnology • Bioassay-guided isolation • Pharmacognosy • Pharmacology of natural products • Metabolomics • Ethnobotany and traditional usage • Genetics of natural products Manuscripts that detail the isolation of just one new compound are not substantial enough to be sent out of review and are out of scope. Furthermore, where pharmacology has been performed on one new compound to increase the amount of novel data, the pharmacology must be substantial and/or related to the medicinal use of the producing organism.
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