Comparison of coagulation and fibrinolysis in Irish Wolfhounds and age-matched control dogs using tissue plasminogen activator-augmented viscoelastic testing

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of veterinary emergency and critical care Pub Date : 2024-05-22 DOI:10.1111/vec.13385

Samantha Davis DVM, Daniel J. Fletcher PhD, DVM, DACVECC, Ashley Newman DVM, Marjory B. Brooks DVM, DACVIM

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引用次数: 0

Abstract

Objective

To determine if Irish Wolfhounds (IWs), like other sighthounds, are hyperfibrinolytic compared with nonsighthound dogs using 2 native and tissue plasminogen activator (tPA)-enhanced viscoelastic assays, one that is whole blood-based (viscoelastic coagulation monitor [VCM]) and the other that is plasma-based thromboelastography (TEG).

Design

Cohort study.

Setting

University teaching hospital.

Animals

A convenience sample of 27 IWs recruited from the Irish Wolfhound Association of New England Specialty and the local community, and 27 healthy, age-matched, large-breed control dogs.

Interventions

None.

Measurements and Main Results

Blood samples including CBC, biochemistry, traditional coagulation, and viscoelastic testing were collected from IWs and control dogs. Twelve IWs had viscoelastic testing. IWs had lower fibrinogen concentrations (215.5 ± 57.8 vs 251.4 ± 64.5 mg/dL, P = 0.034) and formed weaker clots on both whole-blood VCM and plasma TEG assays (maximum clot firmness [VCM-MCF] = 39.4 [25.1–48.8] vs 48.5 [34.6–57.3], P = 0.0042; maximum amplitude [TEG-MA] = 22.7 [14.7–33.6] vs 32.2 [26.9–42.0], P < 0.0001). IWs were hyperfibrinolytic compared with control dogs on VCM whole-blood assays, with 25 U/mL tPA (lysis at 30 min [VCM-LI30] = 68.1 [0–100] vs\ 99.9 [63.3–100], P = 0.0009; lysis at 45 min [VCM-LI45] = 31.0 [0–100] vs 98.1 [38.4–100], P = 0.0002) but hypofibrinolytic compared with controls on TEG plasma assays with 50 U/mL tPA (lysis at 30 min [TEG-LY30] = 45.7 [4.6–94.6] vs 93.7 [12.3–96.5], P = 0.0004; lysis at 60 min [TEG-LY60] = 68.7 [29.7–96.8] vs 95.7 [34.4–97.6], P = 0.0003). Minimal fibrinolysis was measured on whole-blood VCM or plasma TEG assays without the addition of tPA, and there were no differences between the 2 groups.

Conclusions

Weaker clots were found in IWs than control dogs. With the addition of tPA, IWs had evidence of hyperfibrinolysis on whole-blood VCM assays and hypofibrinolysis on plasma TEG assays compared with control dogs. Without the addition of tPA, however, both groups of dogs showed minimal fibrinolysis on viscoelastic testing.

查看原文本刊更多论文

使用组织凝血酶原激活剂增强粘弹性测试，比较爱尔兰猎狼犬和年龄匹配的对照犬的凝血和纤维蛋白溶解情况。

目的：使用两种原生和组织纤溶酶原激活剂（tPA）增强型粘弹性检测方法（一种是基于全血的粘弹性凝血监测仪（VCM），另一种是基于血浆的血栓弹性成像（TEG）），确定爱尔兰猎狼犬（IWs）是否与其他八哥犬一样，具有高纤维蛋白溶解性：设计：队列研究：动物干预措施：无：测量和主要结果从爱尔兰猎狼犬和对照组犬只身上采集血液样本，包括全血细胞计数、生物化学、传统凝血和粘弹性测试。12 只 IW 接受了粘弹性测试。IWs 的纤维蛋白原浓度较低（215.5 ± 57.8 vs 251.4 ± 64.5 mg/dL，P = 0.034），在全血 VCM 和血浆 TEG 检测中形成的凝块较弱（最大凝块坚固度 [VCM-MCF] = 39.4 [25.1-48.8] vs 48.5 [34.6-57.3]，P = 0.0042；最大振幅[TEG-MA] = 22.7 [14.7-33.6] vs 32.2 [26.9-42.0]，P 结论）：与对照组犬相比，IW犬体内发现的血凝块更弱。与对照组犬相比，在添加了 tPA 后，IW 在全血 VCM 检测中显示出高纤维蛋白溶解，而在血浆 TEG 检测中显示出低纤维蛋白溶解。然而，在不添加 tPA 的情况下，两组狗在粘弹性测试中的纤溶程度都很低。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of veterinary emergency and critical care 农林科学-兽医学

CiteScore

2.50

自引率

15.40%

发文量

121

审稿时长

18-36 weeks

期刊介绍： The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.