The effect of anatomic location on porcine models of burn injury and wound healing.

IF 3.8 3区 医学 Q2 CELL BIOLOGY
Wound Repair and Regeneration Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI:10.1111/wrr.13190
Aiping Liu, J Z Alex Cheong, Sameeha Hassan, Matthew B Wielgat, Jennifer J Meudt, Elizabeth Catherine Townsend, Dhanansayan Shanmuganayagam, Lindsay R Kalan, Angela Gibson
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Abstract

Porcine models are frequently used for burn healing studies; however, factors including anatomic location and lack of standardised wound methods can impact the interpretation of wound data. The objectives of this study are to examine the influence of anatomical locations on the uniformity of burn creation and healing in porcine burn models. To optimise burn parameters on dorsal and ventral surfaces, ex vivo and in situ euthanized animals were first used to examine the location-dependence of the burn depth and contact time relationship. The location-dependent healing in vivo was then examined using burn and excisional wounds at dorsal, ventral, caudal and cranial locations. Lactate dehydrogenase (LDH) and H&E were used to assess burn depth and wound re-epithelialization. We found that burn depth on the ventral skin was significantly deeper than that of the dorsal skin at identical thermal conditions. Compared with burns created ex vivo, burns created in situ immediately post-mortem were significantly deeper in the ventral location. In live animals, 2 out of 12 burn wounds were fully re-epithelialized after 14 days in contrast to complete re-epithelialization of all excisional wounds. Among the burn wounds, those at the cranial-dorsal site exhibited faster healing than at the caudal-dorsal site. This study showed that anatomical location is an important consideration for the consistency of burn depth creation and healing. These data support symmetric localization of treatment and control for comparative assessment of burn healing in porcine models to prevent misinterpretation of results and increase the translatability of findings to humans.

解剖位置对猪烧伤和伤口愈合模型的影响。
猪模型经常用于烧伤愈合研究;然而,包括解剖位置和缺乏标准化创面方法在内的因素会影响创面数据的解读。本研究的目的是检验解剖位置对猪烧伤模型烧伤创建和愈合均匀性的影响。为了优化背侧和腹侧表面的烧伤参数,首先使用体外和原位安乐死动物来研究烧伤深度和接触时间关系的位置依赖性。然后,利用背侧、腹侧、尾侧和颅侧的烧伤和切除伤口,对体内的位置依赖性愈合进行了研究。使用乳酸脱氢酶(LDH)和 H&E 评估烧伤深度和伤口再上皮化情况。我们发现,在相同的热条件下,腹侧皮肤的烧伤深度明显深于背侧皮肤。与体内烧伤相比,死后立即在原位烧伤的腹侧皮肤烧伤深度明显更深。在活体动物身上,12 个烧伤创面中有 2 个在 14 天后完全重新上皮,而所有切除创面则完全重新上皮。在烧伤创口中,颅背部位的创口比尾背部位的创口愈合更快。这项研究表明,解剖位置是烧伤深度创建和愈合一致性的一个重要考虑因素。这些数据支持对称定位治疗和对照,以比较评估猪模型的烧伤愈合情况,从而防止对结果的误读,并提高研究结果对人类的可转化性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Wound Repair and Regeneration
Wound Repair and Regeneration 医学-皮肤病学
CiteScore
5.90
自引率
3.40%
发文量
71
审稿时长
6-12 weeks
期刊介绍: Wound Repair and Regeneration provides extensive international coverage of cellular and molecular biology, connective tissue, and biological mediator studies in the field of tissue repair and regeneration and serves a diverse audience of surgeons, plastic surgeons, dermatologists, biochemists, cell biologists, and others. Wound Repair and Regeneration is the official journal of The Wound Healing Society, The European Tissue Repair Society, The Japanese Society for Wound Healing, and The Australian Wound Management Association.
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