Anti-inflammatory effect of proanthocyanidins from blueberry through NF-κβ/NLRP3 signaling pathway in vivo and in vitro.

IF 2.9 4区医学 Q3 IMMUNOLOGY

Immunopharmacology and Immunotoxicology Pub Date : 2024-08-01 Epub Date: 2024-05-30 DOI:10.1080/08923973.2024.2358770

Xinyao Liu, Lulu Zang, Jiabao Yu, Jinjin Yu, Siqi Wang, Lili Zhou, Huixin Song, Yajing Ma, Xiaofeng Niu, Weifeng Li

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引用次数: 0

Abstract

Background: Systemic inflammatory response syndrome (SIRS) is an uncontrolled systemic inflammatory response. Proanthocyanidins (PC) is a general term of polyphenol compounds widely existed in blueberry fruits and can treat inflammation-related diseases. This study aimed to explore the regulatory effect of PC on lipopolysaccharide (LPS)-induced systemic inflammation and its potential mechanism, providing effective strategies for the further development of PC.

Methods: Here, RAW264.7 macrophages were stimulated with LPS to establish an inflammation model in vitro, while endotoxin shock mouse models were constructed by LPS in vivo. The function of PC was investigated by MTT, ELISA kits, H&E staining, immunohistochemistry, and Western blot analysis.

Results: Functionally, PC could demonstrate the potential to mitigate mortality in mice with endotoxin shock, as well as attenuated the levels of inflammatory cytokines (IL-6, TNF-α) and biochemical indicators (AST, ALT, CRE and BUN). Moreover, it had a significant protective effect on lung and kidney tissues damage. Mechanistically, PC exerted anti-inflammatory effects by inhibiting the activation of the NF-κB/NLRP3 signaling pathway.

Conclusion: PC might have the potential ability of anti-inflammatory effects via modulation of the NF-κB/NLRP3 signaling pathway.

查看原文本刊更多论文

蓝莓原花青素通过 NF-κB/NLRP3 信号通路在体内和体外发挥抗炎作用

背景：全身炎症反应综合征（SIRS）是一种不受控制的全身炎症反应。原花青素（PC）是多酚化合物的总称，广泛存在于蓝莓果实中，可治疗炎症相关疾病。本研究旨在探讨PC对脂多糖（LPS）诱导的全身性炎症的调节作用及其潜在机制，为PC的进一步开发提供有效策略。方法：在体外用LPS刺激RAW264.7巨噬细胞建立炎症模型，在体内用LPS构建内毒素休克小鼠模型。通过MTT、ELISA试剂盒、H&E染色、免疫组织化学和Western印迹分析研究了PC的功能：结果：从功能上看，PC具有降低内毒素休克小鼠死亡率的潜力，并能降低炎性细胞因子（IL-6、TNF-α）和生化指标（AST、ALT、CRE和BUN）的水平。此外，它对肺和肾组织损伤也有明显的保护作用。从机理上讲，PC 通过抑制 NF-κB/NLRP3 信号通路的激活而发挥抗炎作用：结论：PC 可通过调节 NF-κB/NLRP3 信号通路发挥潜在的抗炎作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Immunopharmacology and Immunotoxicology 医学-毒理学

CiteScore

5.40

自引率

0.00%

发文量

133

审稿时长

4-8 weeks

期刊介绍： The journal Immunopharmacology and Immunotoxicology is devoted to pre-clinical and clinical drug discovery and development targeting the immune system. Research related to the immunoregulatory effects of various compounds, including small-molecule drugs and biologics, on immunocompetent cells and immune responses, as well as the immunotoxicity exerted by xenobiotics and drugs. Only research that describe the mechanisms of specific compounds (not extracts) is of interest to the journal. The journal will prioritise preclinical and clinical studies on immunotherapy of disorders such as chronic inflammation, allergy, autoimmunity, cancer etc. The effects of small-drugs, vaccines and biologics against central immunological targets as well as cell-based therapy, including dendritic cell therapy, T cell adoptive transfer and stem cell therapy, are topics of particular interest. Publications pointing towards potential new drug targets within the immune system or novel technology for immunopharmacological drug development are also welcome. With an immunoscience focus on drug development, immunotherapy and toxicology, the journal will cover areas such as infection, allergy, inflammation, tumor immunology, degenerative disorders, immunodeficiencies, neurology, atherosclerosis and more. Immunopharmacology and Immunotoxicology will accept original manuscripts, brief communications, commentaries, mini-reviews, reviews, clinical trials and clinical cases, on the condition that the results reported are based on original, clinical, or basic research that has not been published elsewhere in any journal in any language (except in abstract form relating to paper communicated to scientific meetings and symposiums).