Isolation, screening, and characterization of the newly isolated osmotolerant yeast Wickerhamomyces anomalus BKK11-4 for the coproduction of glycerol and arabitol.

IF 2.1 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2024-09-01 Epub Date: 2024-05-22 DOI:10.1007/s42770-024-01383-1
Jesnipit Thammaket, Piroonporn Srimongkol, Paweena Ekkaphan, Sitanan Thitiprasert, Sorapat Niyomsin, Thanyalak Chaisuwan, Suwabun Chirachanchai, Nuttha Thongchul
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Abstract

This study explored the isolation and screening of an osmotolerant yeast, Wickerhamomyces anomalus BKK11-4, which is proficient in utilizing renewable feedstocks for sugar alcohol production. In batch fermentation with high initial glucose concentrations, W. anomalus BKK11-4 exhibited notable production of glycerol and arabitol. The results of the medium optimization experiments revealed that trace elements, such as H3BO3, CuSO4, FeCl3, MnSO4, KI, H4MoNa2O4, and ZnSO4, did not increase glucose consumption or sugar alcohol production but substantially increased cell biomass. Osmotic stress, which was manipulated by varying initial glucose concentrations, influenced metabolic outcomes. Elevated glucose levels promoted glycerol and arabitol production while decreasing citric acid production. Agitation rates significantly impacted the kinetics, enhancing glucose utilization and metabolite production rates, particularly for glycerol, arabitol, and citric acid. The operational pH dictated the distribution of the end metabolites, with glycerol production slightly reduced at pH 6, while arabitol production remained unaffected. Citric acid production was observed at pH 6 and 7, and acetic acid production was observed at pH 7. Metabolomic analysis using GC/MS identified 29 metabolites, emphasizing the abundance of sugar/sugar alcohols. Heatmaps were generated to depict the variations in metabolite levels under different osmotic stress conditions, highlighting the intricate metabolic dynamics occurring post-glucose uptake, affecting pathways such as the pentose phosphate pathway and glycerolipid metabolism. These insights contribute to the optimization of W. anomalus BKK11-4 as a whole-cell factory for desirable products, demonstrating its potential applicability in sustainable sugar alcohol production from renewable feedstocks.

Abstract Image

分离、筛选和鉴定新分离的耐渗透性酵母 Wickerhamomyces anomalus BKK11-4,以共同生产甘油和阿拉伯糖醇。
本研究探索了一种耐渗透酵母 Wickerhamomyces anomalus BKK11-4 的分离和筛选,这种酵母能熟练利用可再生原料生产糖醇。在高初始葡萄糖浓度的批量发酵中,W. anomalus BKK11-4 表现出显著的甘油和阿拉伯糖醇产量。培养基优化实验的结果表明,微量元素(如 H3BO3、CuSO4、FeCl3、MnSO4、KI、H4MoNa2O4 和 ZnSO4)不会增加葡萄糖消耗量或糖醇产量,但会显著增加细胞生物量。通过改变初始葡萄糖浓度来控制的渗透胁迫影响了代谢结果。葡萄糖水平的升高促进了甘油和阿拉伯糖醇的产生,同时降低了柠檬酸的产生。搅拌速率对动力学有重大影响,可提高葡萄糖利用率和代谢物生产率,尤其是甘油、阿拉伯糖醇和柠檬酸的生产率。操作 pH 值决定了最终代谢物的分布,pH 值为 6 时,甘油的产量略有减少,而阿拉伯糖醇的产量则不受影响。利用气相色谱/质谱(GC/MS)进行的代谢组分析确定了 29 种代谢物,强调了糖/糖醇的大量存在。生成的热图描述了不同渗透胁迫条件下代谢物水平的变化,突出显示了葡萄糖吸收后发生的错综复杂的代谢动态,影响了磷酸戊糖途径和甘油酯代谢等途径。这些见解有助于将 W. anomalus BKK11-4 优化为生产理想产品的全细胞工厂,证明了它在利用可再生原料生产可持续糖醇方面的潜在适用性。
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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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