Co-expression and interaction network analysis identifies neutrophil-related genes as the core mediator of atrial fibrillation.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-01 DOI:10.4149/gpb_2024004

Shaolan Liang, Xiaoxue Zhang, Jia Chen, Yongcong He, Jun Lai

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Abstract

Atrial fibrillation (AF) is the most common cardiac arrhythmia and can cause serious complications. Several studies have shown that neutrophils may influence AF progression. However, the key genes related to neutrophils in AF have not been fully elucidated. Here, we downloaded microarray expression data of AF, and screened differentially expressed genes. Key immune cells in AF were identified by immune cell infiltration analysis. Weighted gene co-expression network analysis (WGCNA) and protein-protein interaction (PPI) analysis were used to construct gene co-expression modules and identify hub genes. The association between key genes and neutrophils was then verified. Our results showed that 303 differentially expressed genes (DEGs) were screened in AF and sinus rhythm (SR), of which 194 were up-regulated and 109 were down-regulated. DEGs were mainly enriched in functions and pathways of neutrophil activation and biological functions of neutrophil activation-mediated immune response. Immune infiltration analysis revealed elevated levels of neutrophil infiltration in AF. WGCNA analysis revealed that the modules in dark red were associated with neutrophils. PPI analysis of these modules yielded 10 hub genes. S100A12, FCGR3B and S100A8 are 3 potential key genes related to neutrophils in AF, which are significantly positively correlated with neutrophils. These genes deserve further investigation and may be potential therapeutic targets for AF.

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共表达和相互作用网络分析确定中性粒细胞相关基因是心房颤动的核心介质。

心房颤动（房颤）是最常见的心律失常，可引起严重的并发症。多项研究表明，中性粒细胞可能会影响心房颤动的进展。然而，房颤中与中性粒细胞相关的关键基因尚未完全阐明。在此，我们下载了心房颤动的芯片表达数据，并筛选了差异表达基因。通过免疫细胞浸润分析确定了房颤中的关键免疫细胞。利用加权基因共表达网络分析（WGCNA）和蛋白-蛋白相互作用分析（PPI）构建基因共表达模块并识别枢纽基因。然后验证了关键基因与中性粒细胞之间的关联。结果显示，在房颤和窦性心律（SR）中筛选出了 303 个差异表达基因（DEGs），其中 194 个上调，109 个下调。DEGs主要富集于中性粒细胞活化的功能和通路以及中性粒细胞活化介导的免疫反应的生物学功能。免疫浸润分析显示，房颤患者的中性粒细胞浸润水平升高。WGCNA 分析显示，暗红色的模块与中性粒细胞有关。对这些模块的 PPI 分析得出了 10 个中心基因。S100A12、FCGR3B 和 S100A8 是心房颤动中与中性粒细胞相关的 3 个潜在关键基因，它们与中性粒细胞呈显著正相关。这些基因值得进一步研究，并可能成为心房颤动的潜在治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.