A molecular survey of zoonotic pathogens of public health importance in rodents/shrews and their ectoparasites trapped in Puducherry, India.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-09-02 DOI:10.1093/trstmh/trae033

Brenna Eikenbary, Panneer Devaraju, Aravindasamy Chakkravarthi, Krishan Kumar Sihag, Terence Nathan, Gowdham Thangaraj, Lakshmy Srinivasan, Ashwani Kumar

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Abstract

Background: Globally, India has a high zoonotic disease burden and lacks surveillance data in humans and animals. Rodents are known reservoirs for many zoonotic diseases and their synanthropic behavior poses a great public health threat.

Methods: In this study, trapped rodents/shrews from randomly selected villages within Puducherry, India, and their ectoparasites were screened for zoonotic pathogens, namely, Orientia tsutsugamushi, other pathogenic rickettsiae, Leptospira spp., Cryptosporidium spp., Coxiella burnetii and methicillin-resistant Staphylococcus aureus (MRSA) using conventional PCR. A total of 58 rodents/shrews were trapped from 11 villages. The species trapped were Suncus murinus (49/58, 84.48%), Rattus rattus (8/58, 13.79%) and Rattus norvegicus (1/58, 1.72%). All ectoparasites collected were identified as mites and its infestation rate was 46.55% (27/58).

Results: Real-time PCR targeting the 47 kDa gene of O. tsutsugamushi revealed positivity in one rodent and one shrew (3.45%) and two mite pools (7.41%). Conventional PCR targeting the 56 kDa gene revealed positivity in one shrew and two mite pools and the phylogenetic analysis of all three amplicons indicated the circulation of the Gilliam-related serotype. MRSA was detected in the alimentary tract of a shrew (1/32, 3.13%). Leptospira spp., Rickettsia, Cryptosporidium spp. and Co. burnetii tested negative.

Conclusions: The detection of zoonotic pathogens within reservoir hosts and vectors poses a risk of transmission to humans. This study signifies the need for zoonotic pathogen surveillance in synanthropic rodents/shrews.

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对印度普杜切里捕获的啮齿动物/鼩鼱及其体外寄生虫中具有公共卫生重要性的人畜共患病原体进行分子调查。

背景：在全球范围内，印度的人畜共患病负担沉重，但却缺乏对人类和动物的监测数据。众所周知，啮齿动物是许多人畜共患病的宿主，它们的同类性行为对公共卫生构成了极大的威胁：在这项研究中，使用传统 PCR 技术对印度普度切里随机选择的村庄中捕获的啮齿动物/鼩鼱及其体外寄生虫进行了人畜共患病原体筛查，这些病原体包括恙虫病、其他致病立克次体、钩端螺旋体属、隐孢子虫属、烧伤科克西氏菌和耐甲氧西林金黄色葡萄球菌（MRSA）。共从 11 个村庄诱捕了 58 只啮齿类动物/鼩鼱。诱捕到的啮齿类动物种类有：Suncus murinus（49/58，84.48%）、Rattus rattus（8/58，13.79%）和Rattus norvegicus（1/58，1.72%）。收集到的所有体外寄生虫均被鉴定为螨类，其侵扰率为 46.55%（27/58）：结果：针对恙虫蚊 47 kDa 基因的实时 PCR 检测结果显示，一只啮齿类动物和一只鼩鼱（3.45%）和两只螨虫（7.41%）呈阳性。针对 56 kDa 基因的常规 PCR 在一只鼩鼱和两只螨虫池中发现阳性，所有三个扩增子的系统进化分析表明与 Gilliam 相关的血清型在鼩鼱和两只螨虫池中流行。在一只鼩鼱的消化道中检测到 MRSA（1/32，3.13%）。钩端螺旋体属、立克次体、隐孢子虫属和烧伤蜱的检测结果均为阴性：结论：在水库宿主和载体中检测到人畜共患病原体会给人类带来传播风险。这项研究表明，有必要对同类啮齿动物/鼩鼱进行人畜共患病病原体监测。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.