Approaches to nutritional research using organoids; fructose treatment induces epigenetic changes in liver organoids

IF 4.8 2区 医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Mirai Yamazaki , Hiroya Yamada , Eiji Munetsuna , Yoshitaka Ando , Genki Mizuno , Atsushi Teshigawara , Hayato Ichikawa , Yuki Nouchi , Itsuki Kageyama , Takuya Wakasugi , Hiroaki Ishikawa , Nobutaka Ohgami , Koji Suzuki , Koji Ohashi
{"title":"Approaches to nutritional research using organoids; fructose treatment induces epigenetic changes in liver organoids","authors":"Mirai Yamazaki ,&nbsp;Hiroya Yamada ,&nbsp;Eiji Munetsuna ,&nbsp;Yoshitaka Ando ,&nbsp;Genki Mizuno ,&nbsp;Atsushi Teshigawara ,&nbsp;Hayato Ichikawa ,&nbsp;Yuki Nouchi ,&nbsp;Itsuki Kageyama ,&nbsp;Takuya Wakasugi ,&nbsp;Hiroaki Ishikawa ,&nbsp;Nobutaka Ohgami ,&nbsp;Koji Suzuki ,&nbsp;Koji Ohashi","doi":"10.1016/j.jnutbio.2024.109671","DOIUrl":null,"url":null,"abstract":"<div><p>Nutritional researches have successfully used animal models to gain new insights into nutrient action. However, comprehensive descriptions of their molecular mechanisms of action remain elusive as appropriate <em>in vitro</em> evaluation systems are lacking. Organoid models can mimic physiological structures and reproduce <em>in vivo</em> functions, making them increasingly utilized in biomedical research for a better understand physiological and pathological phenomena. Therefore, organoid modeling can be a powerful approach for to understand the molecular mechanisms of nutrient action. The present study aims to demonstrate the utility of organoids in nutritional research by further investigating the molecular mechanisms responsible for the negative effects of fructose intake using liver organoids. Here, we treated liver organoids with fructose and analyzed their gene expression profiles and DNA methylation levels. Microarray analysis demonstrated that fructose-treated organoids exhibited increased selenoprotein p (<em>Sepp1</em>) gene expression, whereas pyrosequencing assays revealed reduced DNA methylation levels in the <em>Sepp1</em> region. These results were consistent with observations using hepatic tissues from fructose-fed rats. Conversely, no differences in <em>Sepp1</em> mRNA and DNA methylation levels were observed in two-dimensional cells. These results suggest that organoids serve as an ideal <em>in vitro</em> model to recapitulate <em>in vivo</em> tissue responses and help to validate the molecular mechanisms of nutrient action compared to conventional cellular models.</p></div>","PeriodicalId":16618,"journal":{"name":"Journal of Nutritional Biochemistry","volume":null,"pages":null},"PeriodicalIF":4.8000,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutritional Biochemistry","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0955286324001049","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Nutritional researches have successfully used animal models to gain new insights into nutrient action. However, comprehensive descriptions of their molecular mechanisms of action remain elusive as appropriate in vitro evaluation systems are lacking. Organoid models can mimic physiological structures and reproduce in vivo functions, making them increasingly utilized in biomedical research for a better understand physiological and pathological phenomena. Therefore, organoid modeling can be a powerful approach for to understand the molecular mechanisms of nutrient action. The present study aims to demonstrate the utility of organoids in nutritional research by further investigating the molecular mechanisms responsible for the negative effects of fructose intake using liver organoids. Here, we treated liver organoids with fructose and analyzed their gene expression profiles and DNA methylation levels. Microarray analysis demonstrated that fructose-treated organoids exhibited increased selenoprotein p (Sepp1) gene expression, whereas pyrosequencing assays revealed reduced DNA methylation levels in the Sepp1 region. These results were consistent with observations using hepatic tissues from fructose-fed rats. Conversely, no differences in Sepp1 mRNA and DNA methylation levels were observed in two-dimensional cells. These results suggest that organoids serve as an ideal in vitro model to recapitulate in vivo tissue responses and help to validate the molecular mechanisms of nutrient action compared to conventional cellular models.

Abstract Image

利用器官组织进行营养研究的方法;果糖处理诱导肝脏器官组织发生表观遗传学变化。
营养研究成功地利用动物模型获得了营养作用的新见解。然而,由于缺乏适当的体外评估系统,对其分子作用机制的全面描述仍然难以实现。类器官模型可模仿生理结构并再现体内功能,因此越来越多地被用于生物医学研究,以更好地了解生理和病理现象。因此,类器官模型可以成为了解营养素作用分子机制的有力方法。本研究旨在利用肝脏类器官进一步研究果糖摄入负面影响的分子机制,从而证明类器官在营养学研究中的作用。在这里,我们用果糖处理了肝脏器官组织,并分析了它们的基因表达谱和DNA甲基化水平。微阵列分析表明,果糖处理过的器官组织显示硒蛋白p(Sepp1)基因表达增加,而热释光测序分析则显示Sepp1区域的DNA甲基化水平降低。这些结果与使用果糖喂养大鼠的肝组织观察到的结果一致。相反,在二维细胞中没有观察到 Sepp1 mRNA 和 DNA 甲基化水平的差异。这些结果表明,与传统的细胞模型相比,器官组织是再现体内组织反应的理想体外模型,有助于验证营养素作用的分子机制。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Nutritional Biochemistry
Journal of Nutritional Biochemistry 医学-生化与分子生物学
CiteScore
9.50
自引率
3.60%
发文量
237
审稿时长
68 days
期刊介绍: Devoted to advancements in nutritional sciences, The Journal of Nutritional Biochemistry presents experimental nutrition research as it relates to: biochemistry, molecular biology, toxicology, or physiology. Rigorous reviews by an international editorial board of distinguished scientists ensure publication of the most current and key research being conducted in nutrition at the cellular, animal and human level. In addition to its monthly features of critical reviews and research articles, The Journal of Nutritional Biochemistry also periodically publishes emerging issues, experimental methods, and other types of articles.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信