Unveiling the microbial diversity of biofilms on titanium surfaces in full-scale water-cooling plants using metagenomics approach.

IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Folia microbiologica Pub Date : 2024-12-01 Epub Date: 2024-05-21 DOI:10.1007/s12223-024-01170-3
Gayathri Rethinavelu, Rajathirajan Siva Dharshini, Ranjani Manickam, Anandkumar Balakrishnan, Mohandass Ramya, Naga Raju Maddela, Ram Prasad
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引用次数: 0

Abstract

Microbial colonization on the titanium condenser material (TCM) used in the cooling system leads to biofouling and corrosion and influences the water supply. The primary investigation of the titanium condenser was infrequently studied on characterizing biofilm-forming bacterial communities. Different treatment methods like electropotential charge, ultrasonication, and copper coating of titanium condenser material may influence the microbial population over the surface of the titanium condensers. The present study aimed to catalog the primary colonizers and the effect of different treatment methods on the microbial community. CFU (1.7 × 109 CFU/mL) and ATP count (< 5000 × 10-7 relative luminescence units) showed a minimal microbial population in copper-coated surface biofilm as compared with the other treatments. Live and dead cell result also showed consistency with colony count. The biofilm sample on the copper-coated surface showed an increased dead cell count and decreased live cells. In the metagenomic approach, the microbiome coverage was 10.06 Mb in samples derived from copper-coated TCM than in other treated samples (electropotential charge-17.94 Mb; ultrasonication-20.01 Mb), including control (10.18 Mb). Firmicutes preponderate the communities in the biofilm samples, and Proteobacteria stand next in the population in all the treated condenser materials. At the genus level, Lactobacillaceae and Azospirillaceae dominated the biofilm community. The metagenome data suggested that the attached community is different from those biofilm samples based on the environment that influences the bacterial community. The outcome of the present study depicts that copper coating was effective against biofouling and corrosion resistance of titanium condenser material for designing long-term durability.

Abstract Image

利用元基因组学方法揭示大型水冷设备钛表面生物膜的微生物多样性。
冷却系统中使用的钛冷凝器材料(TCM)上的微生物定植会导致生物污垢和腐蚀,并影响供水。对钛冷凝器的初步研究很少涉及生物膜形成细菌群落的特征。钛冷凝器材料的不同处理方法,如电位充电、超声波处理和铜涂层,可能会影响钛冷凝器表面的微生物数量。本研究旨在对主要定殖菌进行编目,并研究不同处理方法对微生物群落的影响。与其他处理方法相比,CFU(1.7 × 109 CFU/mL)和 ATP 计数(-7 相对发光单位)显示铜涂层表面生物膜中的微生物数量极少。活细胞和死细胞结果也与菌落计数一致。铜涂层表面的生物膜样本显示死细胞数增加,活细胞数减少。在元基因组学方法中,与其他处理样本(电位充电-17.94 Mb;超声处理-20.01 Mb)(包括对照组(10.18 Mb))相比,铜涂层中药样本的微生物组覆盖率为 10.06 Mb。在所有处理过的冷凝器样本中,生物膜样本中的菌群以固着菌为主,变形菌次之。在属的层面上,乳杆菌科和氮青杆菌科在生物膜群落中占主导地位。元基因组数据表明,根据影响细菌群落的环境,附着群落与生物膜样本有所不同。本研究的结果表明,铜涂层能有效防止钛冷凝器材料的生物污染和耐腐蚀性,从而设计出长期耐用的冷凝器。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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