Application of UPLC-Q Exactive™ HF-X MS-based untargeted metabolomics reveals variations in Moringa oleifera Lam. leaves from different regions

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochemical Systematics and Ecology Pub Date : 2024-05-17 DOI:10.1016/j.bse.2024.104847

Yanqin Zhu , Le Wang , Qinhong Yin , Jun Yang

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引用次数: 0

Abstract

The metabolites of Moringa oleifera Lam. (M. oleifera) leaves are involved in their biological function. However, a thorough examination of metabolites has not been possible due to the complex nature of metabolite distribution in M. oleifera leaves from different regions. The purpose of this study was to investigate regional differences in M. oleifera leaf metabolite profiles. Leaves from the Dehong, Baoshan, and Yuanmou regions of Yunnan Province were studied using ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-Q Exactive™ HF-X MS)-based untargeted metabolomics. A total of 2084 electrospray ionization-positive compounds and 1294 electrospray ionization-negative compounds were identified. The three sample groups were found to be in different quadrants of the principal component analysis (PCA) map; hierarchical cluster analysis (HCA) indicated that variations in habitat conditions were the cause of the unstable quality of M. oleifera leaves; and Kyoto Encyclopedia of Genes and Genomes (KEGG) and orthogonal partial least squares-discrimination analysis (OPLS-DA) showed regional differences in metabolite numbers and associated pathways. These were the observed variations in the chemical compositions of M. oleifera leaves. Flavonoid and phenylpropanoid biosyntheses were the main focus in pathways related to the biosynthesis of other secondary metabolites. Phenolic substances played a significant role as either leaders or connectors in metabolic pathways. The results provide a deeper understanding of phytochemical variations in M. oleifera leaves from different geographical regions which has potential application in the quality control of raw materials.

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应用 UPLC-Q Exactive™ HF-X MS 非靶向代谢组学揭示不同地区油杉叶片的差异

Moringa oleifera Lam.(油橄榄（M. oleifera）叶片的代谢物与叶片的生物功能有关。然而，由于不同地区油橄榄叶片中代谢物分布的复杂性，还无法对代谢物进行全面研究。本研究旨在调查油橄榄叶片代谢物的地区差异。采用基于超高效液相色谱-串联质谱（UPLC-Q Exactive™ HF-X MS）的非靶向代谢组学方法，对云南省德宏、保山和元谋地区的油橄榄叶片进行了研究。共鉴定出 2084 种电喷雾电离阳性化合物和 1294 种电喷雾电离阴性化合物。结果发现，三个样本组分别位于主成分分析图（PCA）的不同象限；层次聚类分析（HCA）表明，生境条件的变化是油橄榄叶片质量不稳定的原因；京都基因组百科全书（KEGG）和正交偏最小二乘判别分析（OPLS-DA）显示了代谢物数量和相关途径的区域差异。这就是观察到的油橄榄叶化学成分的变化。在与其他次生代谢物的生物合成相关的途径中，类黄酮和苯丙酮的生物合成是重点。酚类物质在代谢途径中扮演着重要的领导者或连接者的角色。研究结果加深了人们对不同地理区域油橄榄叶中植物化学物质变化的了解，这对原材料的质量控制具有潜在的应用价值。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biochemical Systematics and Ecology 生物-进化生物学

CiteScore

3.00

自引率

12.50%

发文量

147

审稿时长

43 days

期刊介绍： Biochemical Systematics and Ecology is devoted to the publication of original papers and reviews, both submitted and invited, in two subject areas: I) the application of biochemistry to problems relating to systematic biology of organisms (biochemical systematics); II) the role of biochemistry in interactions between organisms or between an organism and its environment (biochemical ecology). In the Biochemical Systematics subject area, comparative studies of the distribution of (secondary) metabolites within a wider taxon (e.g. genus or family) are welcome. Comparative studies, encompassing multiple accessions of each of the taxa within their distribution are particularly encouraged. Welcome are also studies combining classical chemosystematic studies (such as comparative HPLC-MS or GC-MS investigations) with (macro-) molecular phylogenetic studies. Studies that involve the comparative use of compounds to help differentiate among species such as adulterants or substitutes that illustrate the applied use of chemosystematics are welcome. In contrast, studies solely employing macromolecular phylogenetic techniques (gene sequences, RAPD studies etc.) will be considered out of scope. Discouraged are manuscripts that report known or new compounds from a single source taxon without addressing a systematic hypothesis. Also considered out of scope are studies using outdated and hard to reproduce macromolecular techniques such as RAPDs in combination with standard chemosystematic techniques such as GC-FID and GC-MS.