Efficacy of mesenchymal stem cell-based therapy on the bone repair of hypertensive rats.

IF 2.9 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Oral diseases Pub Date : 2024-11-01 Epub Date: 2024-05-19 DOI:10.1111/odi.15004

Alann Thaffarell Portilho Souza, Gileade Pereira Freitas, Helena Bacha Lopes, Denise Weffort, Leticia Faustino Adolpho, Maria Paula Oliveira Gomes, Fabiola Singaretti Oliveira, Adriana Luisa Gonçalves Almeida, Marcio Mateus Beloti, Adalberto Luiz Rosa

{"title":"Efficacy of mesenchymal stem cell-based therapy on the bone repair of hypertensive rats.","authors":"Alann Thaffarell Portilho Souza, Gileade Pereira Freitas, Helena Bacha Lopes, Denise Weffort, Leticia Faustino Adolpho, Maria Paula Oliveira Gomes, Fabiola Singaretti Oliveira, Adriana Luisa Gonçalves Almeida, Marcio Mateus Beloti, Adalberto Luiz Rosa","doi":"10.1111/odi.15004","DOIUrl":null,"url":null,"abstract":"Objective: Hypertension disrupts the bone integrity and its repair ability. This study explores the efficiency of a therapy based on the application of mesenchymal stem cells (MSCs) to repair bone defects of spontaneously hypertensive rats (SHR).Methods: First, we evaluated SHR in terms of bone morphometry and differentiation of MSCs into osteoblasts. Then, the effects of the interactions between MSCs from normotensive rats (NTR-MSCs) cocultured with SHR (SHR-MSCs) on the osteoblast differentiation of both cell populations were evaluated. Also, bone formation into calvarial defects of SHR treated with NTR-MSCs was analyzed.Results: Hypertension induced bone loss evidenced by reduced bone morphometric parameters of femurs of SHR compared with NTR as well as decreased osteoblast differentiation of SHR-MSCs compared with NTR-MSCs. NTR-MSCs partially restored the capacity of SHR-MSCs to differentiate into osteoblasts, while SHR-MSCs exhibited a slight negative effect on NTR-MSCs. An enhanced bone repair was observed in defects treated with NTR-MSCs compared with control, stressing this cell therapy efficacy even in bones damaged by hypertension.Conclusion: The use of MSCs derived from a heathy environment can be in the near future a smart approach to treat bone loss in the context of regenerative dentistry for oral rehabilitation of hypertensive patients.","PeriodicalId":19615,"journal":{"name":"Oral diseases","volume":" ","pages":"5118-5128"},"PeriodicalIF":2.9000,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Oral diseases","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1111/odi.15004","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/5/19 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"DENTISTRY, ORAL SURGERY & MEDICINE","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: Hypertension disrupts the bone integrity and its repair ability. This study explores the efficiency of a therapy based on the application of mesenchymal stem cells (MSCs) to repair bone defects of spontaneously hypertensive rats (SHR).

Methods: First, we evaluated SHR in terms of bone morphometry and differentiation of MSCs into osteoblasts. Then, the effects of the interactions between MSCs from normotensive rats (NTR-MSCs) cocultured with SHR (SHR-MSCs) on the osteoblast differentiation of both cell populations were evaluated. Also, bone formation into calvarial defects of SHR treated with NTR-MSCs was analyzed.

Results: Hypertension induced bone loss evidenced by reduced bone morphometric parameters of femurs of SHR compared with NTR as well as decreased osteoblast differentiation of SHR-MSCs compared with NTR-MSCs. NTR-MSCs partially restored the capacity of SHR-MSCs to differentiate into osteoblasts, while SHR-MSCs exhibited a slight negative effect on NTR-MSCs. An enhanced bone repair was observed in defects treated with NTR-MSCs compared with control, stressing this cell therapy efficacy even in bones damaged by hypertension.

Conclusion: The use of MSCs derived from a heathy environment can be in the near future a smart approach to treat bone loss in the context of regenerative dentistry for oral rehabilitation of hypertensive patients.

查看原文本刊更多论文

间充质干细胞疗法对高血压大鼠骨骼修复的疗效。

目的：高血压会破坏骨的完整性及其修复能力。本研究探讨了应用间充质干细胞（MSCs）修复自发性高血压大鼠（SHR）骨缺损的疗法的有效性：首先，我们从骨形态学和间充质干细胞分化成成骨细胞的角度对自发性高血压大鼠进行了评估。然后，评估了正常血压大鼠间充质干细胞（NTR-MSCs）与自发性高血压大鼠间充质干细胞（SHR-MSCs）共同培养对两种细胞群的成骨细胞分化的影响。此外，还分析了用NTR-间充质干细胞处理的SHR腓骨缺损的骨形成情况：结果：高血压诱导的骨质流失表现为 SHR 股骨的骨形态参数比 NTR 降低，以及 SHR 间充质干细胞的成骨细胞分化比 NTR 间充质干细胞降低。NTR-间充质干细胞部分恢复了SHR-间充质干细胞分化成成骨细胞的能力，而SHR-间充质干细胞对NTR-间充质干细胞有轻微的负面影响。与对照组相比，用NTR-间充质干细胞治疗的缺损部位骨修复能力增强，这说明这种细胞疗法即使对高血压造成的骨骼损伤也有疗效：结论：在不久的将来，使用来自健康环境的间充质干细胞治疗高血压患者口腔康复中的再生牙科骨质流失将是一种明智的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Oral diseases 医学-牙科与口腔外科

CiteScore

7.60

自引率

5.30%

发文量

325

审稿时长

4-8 weeks

期刊介绍： Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.