Genome-wide association study and transcriptome analysis reveal natural variation of key genes regulation flowering time in rapeseed

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-05-18 DOI:10.1007/s11032-024-01479-4

Xingru Xiang, Ping Qiu, Zhichao Mei, Min Yao, Wei Liu, Dan He, Song Cao, Xin He, Xinghua Xiong, Zhongsong Liu, Lunwen Qian

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Abstract

Flowering time is a crucial determinant of both the yield and oil quality of rapeseed as well as a key indicator of plant maturity. We performed a genome-wide association study and transcriptome analysis to identify key genes/loci regulating flowering time in Brassica napus L. Forty-six haplotype regions harboring candidate genes were determined to be significantly associated with flowering time, and 28 of these haplotype regions overlapped with previously reported quantitative trait loci. A further investigation of these haplotype regions revealed nucleotide variations in the genes BnaFT-A02, BnaFRI-A10, and BnaFPA-A09 that correlated with phenotypic variations in flowering time. Furthermore, the co-expression network analysis indicated that BnaFT-A02 is directly linked to BnaFRI-A10 and BnaFPA-A09, in a subnetwork and also associated with 13 vernalization, 31 photoperiod, 33 autonomous pathway, and 10 gibberellin pathway genes, forming a potential network regulating flowering time in rapeseed. These results provide valuable haplotype markers for the breeding of early maturing rapeseed varieties.

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全基因组关联研究和转录组分析揭示了调控油菜花期的关键基因的自然变异

开花时间是油菜籽产量和油质的重要决定因素，也是植物成熟度的关键指标。我们进行了一项全基因组关联研究和转录组分析，以确定调控甘蓝型油菜开花时间的关键基因/位点。研究发现，46个单倍型区域中的候选基因与开花时间显著相关，其中28个单倍型区域与之前报道的数量性状位点重叠。对这些单倍型区域的进一步研究发现，基因BnaFT-A02、BnaFRI-A10和BnaFPA-A09的核苷酸变异与花期的表型变异相关。此外，共表达网络分析表明，BnaFT-A02与BnaFRI-A10和BnaFPA-A09直接相连，并与13个春化基因、31个光周期基因、33个自主通路基因和10个赤霉素通路基因相关，形成了一个潜在的油菜开花时间调控网络。这些结果为早熟油菜品种的培育提供了宝贵的单倍型标记。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.