Lecithin Derived Nano-Propyl Gallate as Non-Toxic Anti-Inflammatory Agent: Synthesis, In-Vitro and In-Vivo Investigations

Abstract

The current study describes the preparation of nano-formulation of propyl gallate (NPG vesicles) comprised of soya bean extracted soya-lecithin. The compound was evaluated for its in-vitro and in-vivo anti-oxidative and anti-inflammatory properties in addition with acute toxicity analysis in Wistar rats. Nano-carrier preparation acquired the film hydration method, while the evaluation for size distribution was carried out through dynamic light scattering (DLS) analysis. FTIR spectroscopy was used to identify the interaction between active material with excipient. Whereas, morphological evaluation was carried out by using atomic force microscopy (AFM). UV-visible spectrophotometer was used to measure the efficacy for drug encapsulation. The synthesized nano-carriers of propyl gallate has particle size of 201 ± 2.5 nm, with spherical morphology. The PDI index of nano-formulation is; 0.192 ± 0.8 indicates uniform size distribution with zeta potential − 43.4 ± 1.7mV values representing their highly stable nature. The drug encapsulation efficiency of the NPG vesicles was found to be 52%. The nano-formulation reveals the in-vitro anti-oxidative and anti-inflammatory properties and showed non-toxicity on normal human fibroblast cell line as compared to the parent compound propyl gallate. NPG vesicles showed prominent anti-inflammatory potential against carrageenan induced paw edema and found to be non-toxic in Wistar rats in acute toxicity studies for seven days. In conclusion, nano formulation NPG vesicles showed effective anti-oxidative and anti-inflammatory effects both in-vitro and in-vivo and has the efficacy to become a potential modulator for targeted therapy.