Red and NIR active dipod–SDS self-assemblies for “turn on” quantification of spermine in serum, urine and food: smart-phone assisted on-site determination of spermine in amine-rich foods†

IF 3.5 Q2 CHEMISTRY, ANALYTICAL
Nancy Singla, Sukhvinder Dhiman, Manzoor Ahmad, Satwinderjeet Kaur, Prabhpreet Singh and Subodh Kumar
{"title":"Red and NIR active dipod–SDS self-assemblies for “turn on” quantification of spermine in serum, urine and food: smart-phone assisted on-site determination of spermine in amine-rich foods†","authors":"Nancy Singla, Sukhvinder Dhiman, Manzoor Ahmad, Satwinderjeet Kaur, Prabhpreet Singh and Subodh Kumar","doi":"10.1039/D3SD00300K","DOIUrl":null,"url":null,"abstract":"<p >Spermine is a vital biomarker for clinical diagnosis of cancer and estimating food spoilage. Here, supramolecular assemblies of two donor–π–acceptor dipods <strong>R-SPM</strong> (<em>λ</em><small><sub>em</sub></small> 640 nm) and <strong>NIR-SPM</strong> (<em>λ</em><small><sub>em</sub></small> 720 nm) with SDS have been discovered for the detection of spermine and spermidine under physiological conditions at nanomolar levels. The addition of SDS to <strong>R-SPM</strong> and <strong>NIR-SPM</strong> results in the formation of self-assemblies (DLS, zeta-potential and UV-vis studies) with no significant change in their fluorescence but further addition of spermine/spermidine to the <strong>R-SPM</strong>∩<strong>SDS</strong> and <strong>NIR-SPM</strong>∩<strong>SDS</strong> assemblies results in a 30–80 fold increase in fluorescence intensity, respectively at 640 nm and 720 nm. The LOD for spermine and spermidine detection is 22 nM (4.4 ppb) and 67 nM (9.7 ppb). The ensembles show nominal interference from other biogenic amines, amino acids, metal ions, and anions. Both <strong>R-SPM</strong>∩<strong>SDS</strong> and <strong>NIR-SPM</strong>∩<strong>SDS</strong> ensembles can be stored in the dark for &gt;3 months without affecting their performance. The potential of these ensembles for real world applications like analysis of spermine in urine, human serum and food spoilage in the case of cheese, mushrooms, chicken and mutton has been demonstrated. The smartphone relied RGB analysis facilitates the on-site determination of spermine in food samples.</p>","PeriodicalId":74786,"journal":{"name":"Sensors & diagnostics","volume":null,"pages":null},"PeriodicalIF":3.5000,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.rsc.org/en/content/articlepdf/2024/sd/d3sd00300k?page=search","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sensors & diagnostics","FirstCategoryId":"1085","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2024/sd/d3sd00300k","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

Abstract

Spermine is a vital biomarker for clinical diagnosis of cancer and estimating food spoilage. Here, supramolecular assemblies of two donor–π–acceptor dipods R-SPM (λem 640 nm) and NIR-SPM (λem 720 nm) with SDS have been discovered for the detection of spermine and spermidine under physiological conditions at nanomolar levels. The addition of SDS to R-SPM and NIR-SPM results in the formation of self-assemblies (DLS, zeta-potential and UV-vis studies) with no significant change in their fluorescence but further addition of spermine/spermidine to the R-SPMSDS and NIR-SPMSDS assemblies results in a 30–80 fold increase in fluorescence intensity, respectively at 640 nm and 720 nm. The LOD for spermine and spermidine detection is 22 nM (4.4 ppb) and 67 nM (9.7 ppb). The ensembles show nominal interference from other biogenic amines, amino acids, metal ions, and anions. Both R-SPMSDS and NIR-SPMSDS ensembles can be stored in the dark for >3 months without affecting their performance. The potential of these ensembles for real world applications like analysis of spermine in urine, human serum and food spoilage in the case of cheese, mushrooms, chicken and mutton has been demonstrated. The smartphone relied RGB analysis facilitates the on-site determination of spermine in food samples.

Abstract Image

Abstract Image

用于 "开启 "血清、尿液和食品中精胺定量的红色和近红外活性二极体-SDS 自组装:智能手机辅助现场测定富含胺的食品中的精胺
精胺是一种重要的生物标志物,可用于癌症的临床诊断和食品腐败的评估。在这里,我们发现了两种供体-π-受体二极体 R-SPM(λem 640 nm)和 NIR-SPM(λem 720 nm)与 SDS 的超分子组装体,可在生理条件下检测纳摩尔水平的精胺和亚精胺。在 R-SPM 和 NIR-SPM 中加入 SDS 会形成自组装(DLS、zeta 电位和 UV-vis 研究),其荧光没有显著变化,但在 R-SPM∩SDS 和 NIR-SPM∩SDS 组装中进一步加入精胺/亚精胺会使 640 纳米和 720 纳米波长处的荧光强度分别增加 30-80 倍。精胺和亚精胺的检测限分别为 22 nM(4.4 ppb)和 67 nM(9.7 ppb)。组合物显示,其他生物胺、氨基酸、金属离子和阴离子的干扰很小。R-SPM∩SDS 和 NIR-SPM∩SDS 组合可在黑暗中保存 3 个月而不影响其性能。这些组件在实际应用中的潜力已得到证实,如分析尿液中的精胺、人体血清以及奶酪、蘑菇、鸡肉和羊肉中的食品腐败。依靠智能手机进行的 RGB 分析有助于现场测定食品样本中的精胺。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.30
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信