Aquaporin expression in cryopreserved human sperm: exploring the capabilities of natural deep eutectic solvents (NADEs).

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-01 DOI:10.54680/fr24310110512

Fatemeh Jahanseir, F. Ghasemian, Z. Zahiri

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引用次数: 0

Abstract

BACKGROUND Aquaporins (AQPs) are essential proteins that facilitate the rapid movement of water and cryoprotective agents (CPAs) during the cryopreservation process, and ensure the cryo-tolerance of sperm cells. OBJECTIVE This study evaluated the preservation of aquaporin levels in human sperm after undergoing freezing using natural deep eutectic solvents (NADES) as CPAs for cryoprotection. MATERIALS AND METHODS From June 2021 to October 2022, 35 semen samples with normal sperm parameters were acquired from the Mehr Infertility Treatment Institute in Rasht, Iran. The samples were divided into several groups for analysis: control group (not frozen), group frozen with SpermFreeze Solution, and groups frozen with different NADESs, including ChS, ChX, ChU, ChG, GlyP, and EtP. After thawing, various aspects for each group were assessed, including the integrity and condensation of sperm chromatin, viability, motility, integrity of acrosome, and the expression of AQP1, AQP3, AQP7, AQP8, and AQP9 genes. RESULTS The analysis of gene expression revealed that freezing with ChS and GlyP preserved the expression of the AQP1 and AQP3 genes compared to the control group. Regarding AQP7 and AQP8, significant differences were not observed in expression levels between certain NADES groups (e.g., ChS, ChU, and GlyP) and the control group. Additionally, samples frozen with specific NADESs, such as ChS, ChG, EtP, and GlyP, exhibited preserved levels of AQP9 expression when compared to the control group. CONCLUSION These findings emphasize the importance of NADES in preserving the expression of aquaporins in cryopreserved human sperm and their important fertility parameters. Doi.org/10.54680/fr24310110512.

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冷冻保存的人类精子中的水蒸发蛋白表达：探索天然深共晶溶剂（NADEs）的能力。

背景水蒸发蛋白（AQPs）是一种重要的蛋白质，可在冷冻保存过程中促进水和冷冻保护剂（CPAs）的快速移动，并确保精子细胞的冷冻耐受性。材料与方法从 2021 年 6 月到 2022 年 10 月，伊朗拉什特 Mehr 不育症治疗研究所共采集了 35 份精子参数正常的精液样本。这些样本被分成几组进行分析：对照组（未冷冻）、用精子冷冻液冷冻组和用不同的 NADES（包括 ChS、ChX、ChU、ChG、GlyP 和 EtP）冷冻组。结果基因表达分析表明，与对照组相比，用 ChS 和 GlyP 冻结的组保留了 AQP1 和 AQP3 基因的表达。至于 AQP7 和 AQP8，某些 NADES 组（如 ChS、ChU 和 GlyP）与对照组的表达水平没有明显差异。此外，与对照组相比，用特定 NADES（如 ChS、ChG、EtP 和 GlyP）冷冻的样本显示出 AQP9 表达水平的保留。Doi.org/10.54680/fr24310110512.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.