Alterations in the pro-resolving lipid mediator machinery within first trimester maternal tissue: Implications in decidualization and miscarriage risk

IF 3
Luísa G. Sousa , Patrícia Alves , Natércia Teixeira , Georgina Correia-da-Silva , Bruno M. Fonseca
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Abstract

A pivotal event in uterine receptivity and human reproduction is the differentiation of endometrial stromal cells into decidual cells, known as decidualization. Decidualization is interlinked with its inflammatory environment. Our study aimed to investigate the presence and role of pro-resolving lipid mediators in first trimester maternal tissue. We assessed the levels of LXA4 and RvD1, along with their metabolic LOX enzymes, in elective (control) and sporadic miscarriage samples. We investigated the effects of LXA4 and RvD1 on decidualization using primary endometrial stromal cells and the immortalized endometrial stromal St-T1b cell line. The upregulation of 12- and 15-LOX expression was observed in pregnancy tissue after sporadic miscarriage, suggesting an inflammatory imbalance. Furthermore, incubation with these lipid mediators led to a decrease in decidualization biomarkers PRL and IGFBP-1, accompanied by morphological changes indicative of aberrant differentiation. The expression of LOX enzymes in decidual natural killer cells suggests their involvement in regulating the inflammatory surroundings and the extent of decidualization.

Abstract Image

妊娠头三个月母体组织中的促溶解脂质介质机制的变化:对蜕膜化和流产风险的影响
子宫受孕和人类生殖的一个关键事件是子宫内膜基质细胞分化为蜕膜细胞,即蜕膜化。蜕膜化与其炎症环境相互关联。我们的研究旨在调查促进蜕膜化的脂质介质在妊娠头三个月母体组织中的存在和作用。我们评估了选择性流产(对照组)和散发性流产样本中 LXA4 和 RvD1 及其代谢 LOX 酶的水平。我们利用原代子宫内膜基质细胞和永生化子宫内膜基质 St-T1b 细胞系研究了 LXA4 和 RvD1 对蜕膜化的影响。在散发性流产后的妊娠组织中观察到 12-LOX 和 15-LOX 表达上调,这表明炎症失衡。此外,与这些脂质介质一起孵育会导致蜕膜化生物标志物 PRL 和 IGFBP-1 的减少,并伴随着表明异常分化的形态学变化。LOX酶在蜕膜自然杀伤细胞中的表达表明,它们参与了炎症环境和蜕膜化程度的调节。
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来源期刊
Prostaglandins, leukotrienes, and essential fatty acids
Prostaglandins, leukotrienes, and essential fatty acids Clinical Biochemistry, Endocrinology, Diabetes and Metabolism
CiteScore
5.30
自引率
0.00%
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0
审稿时长
64 days
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