Selma B. Belfakir , Winnie E. Svendsen , Andreas H. Laustsen , Georgina M.S. Ross
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引用次数: 0
Abstract
Paper-based analytical devices (PADs) enable the affordable, easy, rapid, and reliable detection of a range of analytes at the point-of-care. Cellulose is a versatile substrate for antibody attachment. However, how immunoreagents are immobilized onto cellulose plays an important role in assay performance. To provide an overview of the different antibody immobilization strategies used for developing PADs, we here critically review the existing literature from the last decade (2013–2023). First, we introduce cellulose as a substrate and summarize the different fabrication techniques for developing PADs. Thereafter, we delve into how antibodies are immobilized onto cellulose passively, covalently, or by affinity linkage. Affinity-based immobilization is further explored in a case study focused on using recombinant bifunctional proteins with cellulose-binding modules to anchor immunoreagents to cellulose. The final section discusses the validation and sustainable development of PADs addressing the existing and future challenges associated with developing such devices.
纸质分析装置(PAD)可在医疗点对一系列分析物进行经济、简便、快速和可靠的检测。纤维素是抗体附着的通用底物。然而,如何将免疫试剂固定在纤维素上对检测性能起着重要作用。为了概述用于开发 PAD 的不同抗体固定策略,我们在此对过去十年(2013-2023 年)的现有文献进行了批判性回顾。首先,我们介绍了作为基质的纤维素,并总结了用于开发 PAD 的不同制备技术。随后,我们深入探讨了抗体是如何被动、共价或通过亲和连接固定在纤维素上的。在一项案例研究中,我们进一步探讨了基于亲和力的固定化技术,重点是使用带有纤维素结合模块的重组双功能蛋白将免疫试剂锚定在纤维素上。最后一部分讨论了 PAD 的验证和可持续发展问题,以应对开发此类设备所面临的现有和未来挑战。
期刊介绍:
TrAC publishes succinct and critical overviews of recent advancements in analytical chemistry, designed to assist analytical chemists and other users of analytical techniques. These reviews offer excellent, up-to-date, and timely coverage of various topics within analytical chemistry. Encompassing areas such as analytical instrumentation, biomedical analysis, biomolecular analysis, biosensors, chemical analysis, chemometrics, clinical chemistry, drug discovery, environmental analysis and monitoring, food analysis, forensic science, laboratory automation, materials science, metabolomics, pesticide-residue analysis, pharmaceutical analysis, proteomics, surface science, and water analysis and monitoring, these critical reviews provide comprehensive insights for practitioners in the field.