Cryo-storage of porcine hides at the industrial scale for tissue engineering and regenerative medicine application.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-01 DOI:10.54680/fr24310110312

Huidan Wang, Senli Huang, Yuting Tang, Wendell Q. Sun

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引用次数: 0

Abstract

BACKGROUND The industrial scale cryo-storage of raw tissue materials requires a robust, low-cost and easy-to-operate method that can facilitate the down-stream process. OBJECTIVE The study was aimed to develop the multifunctional protective solutions (MPS) for transportation at ambient conditions and also subsequent cryo-storage below -20 degree C of raw porcine hides for tissue engineering and regenerative medicine. MATERIALS AND METHODS Protective solutions with antimicrobial activity and proteinase-inhibiting activity were developed and tested for its efficacy in preserving the extracellular matrix of porcine dermis from microbial spoilage, proteolytic degradation, freeze damage and excessive dehydration during shipping and cryo-storage. The MPSs contained phosphate-buffered saline with ethylene diamine tetra acetic acid (EDTA) added as chelator and proteinase inhibitor, as well as glycerol or maltodextrin (M180) as cryoprotectants. RESULTS MPSs prepared with EDTA and glycerol or M180 had significant antimicrobial activity and proteinase-inhibiting activity during the period of shipping and handling. Glycerol and M180 prevented eutectic salt precipitation and excessive freeze dehydration upon cryo-storage of porcine hides. Without glycerol or M180, hides could be freeze-dehydrated to the low hydration at ~0.4 g/g dw, and formed irreversible plications after freezing. A critical hydration (0.8~0.9 g/g dw) was observed for the extracellular matrix of porcine dermis, and dehydration to a lower level could impose enormous stress and potential damage. The soaking of porcine hides in MPSs decreased water content as glycerol and M180 entered into dermis. Upon equilibration, the glycerol content in the tissue was about 94% of the incubating glycerol solution, but the M180 content in the tissue was only about 50% of the incubating M180 solution, indicating that M180 did not get into the entire aqueous domain within dermis. MPSs reduced ice formation and increased the unfrozen water content of porcine raw hides upon cryo-storage. CONCLUSION MPSs prepared with EDTA and glycerol or M180 have antimicrobial activity and proteinase-inhibiting activity, which can be used for transportation and cryo-storage of raw hides at the industrial scale. Glycerol at 7.5% w/v and M180 at 20% w/v were sufficient to prevent freeze damage and excessive freeze dehydration. Doi.org/10.54680/fr24310110312.

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工业规模的猪皮冷冻储存，用于组织工程和再生医学应用。

研究旨在开发多功能保护溶液（MPS），用于在环境条件下运输以及随后在零下 20 摄氏度以下低温储存用于组织工程和再生医学的生猪皮。材料与方法开发了具有抗菌活性和蛋白酶抑制活性的保护溶液，并测试了其在运输和冷冻储存过程中保护猪真皮细胞外基质免受微生物腐败、蛋白水解降解、冷冻损伤和过度脱水的功效。结果用乙二胺四乙酸（EDTA）和甘油或麦芽糊精（M180）制备的 MPS 在运输和处理期间具有显著的抗菌活性和蛋白酶抑制活性。甘油和 M180 可防止猪皮冷冻储存时出现共晶盐沉淀和过度冷冻脱水。如果没有甘油或 M180，猪皮可能会冷冻脱水至 ~0.4 g/g dw 的低水合度，并在冷冻后形成不可逆的板块。据观察，猪真皮的细胞外基质有一个临界水合度（0.8~0.9 g/g dw），脱水到较低水平会造成巨大压力和潜在损害。将猪皮浸泡在 MPSs 中，随着甘油和 M180 进入真皮层，水分含量降低。平衡后，组织中的甘油含量约为培养的甘油溶液的 94%，但组织中的 M180 含量仅为培养的 M180 溶液的 50%，这表明 M180 并未进入真皮内的整个水域。用 EDTA 和甘油或 M180 制备的 MPS 具有抗菌活性和蛋白酶抑制活性，可用于工业规模的生皮运输和冷冻储存。7.5% w/v 的甘油和 20% w/v 的 M180 足以防止冻害和过度冷冻脱水。Doi.org/10.54680/fr24310110312.

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.