A comprehensive integration of factors affecting vitamin B12 concentration in milk of Holstein cows: Genetic variability, milk productivity, animal characteristics, and feeding management

Abstract

Daily vitamin B₁₂ (VB₁₂) requirements of humans can naturally be fulfilled by animal product consumption, especially products from ruminants because of bacteria dwelling in their rumen. Indeed, only bacteria can synthesize this vitamin. Milk is hence an excellent source of VB₁₂. This cross-sectional study was undertaken to unravel factors, such as genetic variation, diet and cow characteristics, and milk production, explaining the large variation in milk VB₁₂ concentration among cows by using an integrative approach. Milk samples from 2 consecutive milkings were collected from 3,533 Canadian Holstein cows (1,239 first, 932 second, and 1,362 third and more lactations) located in 99 herds with various feeding management. For the purpose of genetic variation analysis, pedigrees were traced back for 3 complete generations for each sire and dam. A total of 10,021 identities were used in the subsequent genetic analyses. Milk VB₁₂ averaged 4.2 ng/mL with a range between 0.7 and 9.0 ng/mL. Dietary fiber (NDF from forage, dietary NDF, ADF, and lignin) increased and dietary components related to energy (NFC, starch, NE_L, and percentage of concentrate) decreased VB₁₂ in milk. Milk VB₁₂ varied with DIM, with a similar pattern as milk fat and protein concentration lactation curves. Milk VB₁₂ increased as age at calving increased. When disregarding the herd variance, the heritability value was 0.37, meaning that milk VB₁₂ can be modified by genetic selection. The final model including factors related to the diet, animal characteristics and milk productivity, and genetic variation explained 79% (pseudo-R²) of the milk VB₁₂ variation. When excluding the random effect of the cow (i.e., excluding the animal and genetic relationships), the pseudo-R² dropped to 43%, reinforcing the importance of genetic variation in explaining milk VB₁₂ variation. To our knowledge, the present study is the most comprehensive evaluation of factors affecting milk VB₁₂ variation including the greatest number of cows from various lactation stages.