Schauerella fraxinea gen. nov., sp. nov., a bacterial species that colonises ash trees tolerant to dieback caused by Hymenoscyphus fraxineus

IF 3.3 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Undine Behrendt , Valentin Burghard , Sonja Wende , Kristina Ulrich , Jacqueline Wolf , Meina Neumann-Schaal , Andreas Ulrich
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Abstract

The tolerance of ash trees against the pathogen Hymenoscyphus fraxineus seems to be associated with the occurrence of specific microbial taxa on leaves. A group of bacterial isolates, primarily identified on tolerant trees, was investigated with regard to their taxonomic classification and their potential to suppress the ash dieback pathogen. Examination of OGRI values revealed a separate species position. A phylogenomic analysis, based on orthologous and marker genes, indicated a separate genus position along with the species Achromobacter aestuarii. Furthermore, analysis of the ratio of average nucleotide identities and genome alignment fractions demonstrated genomic dissimilarities typically observed for inter-genera comparisons within this family. As a result of these investigations, the strains are considered to represent a separate species within a new genus, for which the name Schauerella fraxinea gen. nov., sp. nov. is proposed, with the type strain B3P038T (=LMG 33092 T = DSM 115926 T). Additionally, a reclassification of the species Achromobacter aestuarii as Schauerella aestuarii comb. nov. is proposed.

In a co-cultivation assay, the strains were able to inhibit the growth of a H. fraxineus strain. Accordingly, a functional analysis of the genome of S. fraxinea B3P038T revealed genes mediating the production of antifungal substances. This potential, combined with the prevalent presence in the phyllosphere of tolerant ash trees, makes this group interesting for an inoculation experiment with the aim of controlling the pathogen in an integrative approach. For future field trials, a strain-specific qPCR system was developed to establish an efficient method for monitoring the inoculation success.

Schauerella fraxinea gen.
白蜡树对病原 Hymenoscyphus fraxineus 的耐受性似乎与叶片上出现的特定微生物类群有关。研究人员对一组细菌分离物(主要在耐受性强的树木上发现)进行了调查,以了解它们的分类学分类及其抑制白蜡枯病原体的潜力。对 OGRI 值的研究发现了一个独立的物种位置。基于同源基因和标记基因的系统发生组分析表明,Achromobacter aestuarii 和 Achromobacter aestuarii 属于不同的属。此外,对平均核苷酸相同度比率和基因组比对分数的分析表明,在该科属间比较中通常会观察到基因组差异。根据这些研究结果,认为这些菌株代表了一个新属中的一个独立种,并将其命名为 Schauerella fraxinea gen.此外,还建议将 Achromobacter aestuarii 重新分类为 Schauerella aestuarii comb.因此,对 S. fraxinea B3P038T 基因组的功能分析发现了介导产生抗真菌物质的基因。这种潜力,再加上白蜡树叶球中普遍存在的耐受性,使该菌株组对接种实验产生了兴趣,目的是以综合方法控制病原体。针对未来的实地试验,我们开发了一种菌株特异性 qPCR 系统,以建立一种监测接种成功率的有效方法。
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来源期刊
Systematic and applied microbiology
Systematic and applied microbiology 生物-生物工程与应用微生物
CiteScore
7.50
自引率
5.90%
发文量
57
审稿时长
22 days
期刊介绍: Systematic and Applied Microbiology deals with various aspects of microbial diversity and systematics of prokaryotes. It focuses on Bacteria and Archaea; eukaryotic microorganisms will only be considered in rare cases. The journal perceives a broad understanding of microbial diversity and encourages the submission of manuscripts from the following branches of microbiology:
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