High expression of serine protease inhibitor kazal type 1 predicts poor prognosis and promotes the progression and invasion of oral tongue squamous cell carcinoma

IF 2.2 4区 医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE
Shuang Wang , Yaping Sun , Dan Shao , Yunjie Pan , Xiaoyan Gao , Peng Zhao , Qiaoling Liu , Gaishuang Shang , Wei Shang , Zhiguang Fu , Yong Sun
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引用次数: 0

Abstract

Objective

This study aimed to investigate the expression of serine protease inhibitor kazal type 1 (SPINK1) and its carcinogenic effect in oral tongue squamous cell carcinoma (OTSCC). Design: Initially, bioinformatics analysis was conducted using data from The Cancer Genome Atlas and Gene Expression Omnibus to compare SPINK1 mRNA expression between malignant and adjacent tissues. Subsequently, the impact of differential expression on survival and other clinical variables was examined. Additionally, histology microarray analysis was performed to assess SPINK1 protein expression in 35 cases of malignant and adjacent tissues. Finally, alterations in SPINK1 expression were evaluated to determine its biological phenotypes in OTSCC, including proliferation, apoptosis, invasion, and metastasis. Results: OTSCC tissues exhibit higher levels of SPINK1 compared to surrounding cancerous tissues. Notably, increased SPINK1 expression correlates with the pathological N stage and independently predicts overall survival among patients with OTSCC. Conclusion: Suppression of SPINK1 inhibited OTSCC cell proliferation, invasion, and motility while promoting apoptosis. These findings suggest that SPINK1 may serve as a prognostic biomarker as well as a potential therapeutic target for managing OTSCC.

丝氨酸蛋白酶抑制剂 kazal 1 型的高表达可预测口腔舌鳞状细胞癌的不良预后并促进其发展和侵袭
目的 本研究旨在探讨丝氨酸蛋白酶抑制剂卡扎尔1型(SPINK1)在口腔舌鳞状细胞癌(OTSCC)中的表达及其致癌作用。研究设计首先,利用癌症基因组图谱(The Cancer Genome Atlas)和基因表达总库(Gene Expression Omnibus)中的数据进行生物信息学分析,比较恶性组织和邻近组织中 SPINK1 mRNA 的表达情况。随后,研究人员考察了不同表达对生存率和其他临床变量的影响。此外,还进行了组织学芯片分析,以评估35例恶性肿瘤和邻近组织的SPINK1蛋白表达情况。最后,通过评估 SPINK1 表达的变化,确定其在 OTSCC 中的生物学表型,包括增殖、凋亡、侵袭和转移。结果与周围癌组织相比,OTSCC组织的SPINK1水平更高。值得注意的是,SPINK1表达的增加与病理N分期相关,并能独立预测OTSCC患者的总生存期。结论抑制SPINK1可抑制OTSCC细胞的增殖、侵袭和运动,同时促进细胞凋亡。这些研究结果表明,SPINK1可作为预后生物标志物以及治疗OTSCC的潜在靶点。
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来源期刊
Archives of oral biology
Archives of oral biology 医学-牙科与口腔外科
CiteScore
5.10
自引率
3.30%
发文量
177
审稿时长
26 days
期刊介绍: Archives of Oral Biology is an international journal which aims to publish papers of the highest scientific quality in the oral and craniofacial sciences. The journal is particularly interested in research which advances knowledge in the mechanisms of craniofacial development and disease, including: Cell and molecular biology Molecular genetics Immunology Pathogenesis Cellular microbiology Embryology Syndromology Forensic dentistry
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