Evaluation of functional properties of lactic acid bacteria isolated from traditional cheese

Sarhan Mohammed, Nilgün Özdemir, Ahmet Hilmi Çon
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Abstract

The objective of this research was to study a total of 10 strains of lactic acid bacteria previously isolated from traditional cheese, explore the effects of various in vitro tests, and evaluate their functional and potential probiotic properties. We tested the strains for cholesterol assimilation, antimicrobial activity, enzyme and exopolysaccharides (EPS) production, cell surface traits, antibiofilm, and tolerance to lysozyme and phenol. E. faecalis S135 and E. durans S1121 were characterized by strong antibacterial activities against C. albicans and S. aureus. The strains E. durans S092 and E. faecalis S135 were differentiated by phytase-producing and coagulation activity, respectively. Moreover, E. faecalis S135 had a greater cholesterol reduction percentage (66.6 ± 0.00 %) than the others. Separately, E. durans S104 and L. coryniformis subsp. torquens S232 were found to possess stronger cell surface properties, e.g., auto-aggregation (94.3 ± 0.30 %) and cell surface hydrophobicity (65.1 ± 0.11 %). All LAB 10 strains were able to handle 0.4 % phenol and 2 mg/mL lysozyme well. They also had some antibiofilm activity, but not much EPS production. Industrially important strains with antimicrobial activity, such as E. durans (S1121, S092, and S104) and E. faecalis S135, could serve as bio-protective agents. These four strains can therefore find application in various fields, including the production of fermented and/or functional foods and enzymes.

评估从传统奶酪中分离出来的乳酸菌的功能特性
这项研究的目的是研究以前从传统奶酪中分离出来的总共 10 株乳酸菌,探索各种体外测试的效果,并评估它们的功能和潜在益生特性。我们测试了这些菌株的胆固醇同化、抗菌活性、酶和外多糖(EPS)的产生、细胞表面特征、抗生物膜以及对溶菌酶和苯酚的耐受性。E. faecalis S135 和 E. durans S1121 对白色念珠菌和金黄色葡萄球菌具有很强的抗菌活性。E. durans S092 和 E. faecalis S135 菌株分别具有产生植酸酶和凝结活性。此外,粪肠球菌 S135 的胆固醇降低率(66.6 ± 0.00 %)高于其他菌株。另外,发现 E. durans S104 和 L. coryniformis subsp. torquens S232 具有更强的细胞表面特性,如自动聚集(94.3 ± 0.30 %)和细胞表面疏水性(65.1 ± 0.11 %)。所有 LAB 10 菌株都能很好地处理 0.4 % 苯酚和 2 mg/mL 溶菌酶。它们还具有一定的抗生物膜活性,但EPS产量不多。具有抗菌活性的重要工业菌株,如杜氏大肠杆菌(S1121、S092 和 S104)和粪大肠杆菌 S135,可作为生物保护剂。因此,这四种菌株可应用于多个领域,包括生产发酵和/或功能性食品和酶。
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