Evaluation of the Protective and Healing Effects of Heracleum lasiopetalum Boiss on a Modified Acetic Acid-Induced Ulcerative Colitis Model in Rats

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-05 DOI:10.5812/jjnpp-143801

H. Malekshahi, G. Bahrami, Atefeh Babaei, Shahram Miraghaee, Nastaran Jalilian, Seyed Hamid Madani, Mohammad Hossain Farzaei

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引用次数: 0

Abstract

Background: Ulcerative colitis (UC), a chronic inflammatory condition of the colon with no definitive cure, necessitates the exploration of innovative therapies. Herbal remedies combined with conventional drugs may offer complementary benefits, enhancing treatment outcomes. Objectives: This study investigates the therapeutic effects of Heracleum lasiopetalum Boiss in a rat model of acetic acid-induced UC. Methods: Forty-eight adult female Wistar rats were randomly allocated to six groups (n = 8 per group) for each of two experiments. UC was induced by intracolonic administration of 1 ml of 4% acetic acid for 10 minutes. In the treatment groups, female rats received an oral gavage of 5%, 10%, and 40% aqueous plant extract, sulfasalazine (100 mg/kg), and distilled water for 6 days, starting 3 days after acetic acid administration. The protective groups received substances via oral gavage from 7 days before to 3 days after acetic acid administration. The extent of mucosal ulcers, hyperemia, inflammation, and mucosal bleeding was evaluated based on the Gerald Classification System Score (Macroscopic). Pathological assessment was conducted on prepared slides using the modified Wallace method (Microscopic). Changes in body and colon weight, along with food and water intake, were examined. Results: Significant changes in food intake were observed in both the extract and positive control treatment groups, exhibiting a notable difference compared to the negative control group (P < 0.05). Body weight changes experienced a significant increase in the positive control treatment group compared to the negative control group (P < 0.05), while no significant difference was evident between the extract and negative control treatment groups (P > 0.05). In terms of water and food intake, as well as weight changes, no significant differences were detected among the various protective groups (P > 0.05). Both microscopic and macroscopic examinations revealed a substantial enhancement in the colon of the extract and positive control treatment groups compared to the negative control group (P < 0.05), with no significant difference observed between the protective groups (P > 0.05). Conclusions: Heracleum lasiopetalum extract demonstrates a dose-dependent reduction in colon hyperemia, mucosal ulceration, inflammation, and fibrosis. This highlights its potential as a supplementary treatment option for UC, emphasizing the need for further investigation.

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评估白花蛇舌草对改良醋酸诱导的大鼠溃疡性结肠炎模型的保护和治疗作用

背景：溃疡性结肠炎（UC）是一种无法根治的慢性结肠炎，因此有必要探索创新疗法。草药疗法与传统药物相结合，可起到互补作用，提高治疗效果。研究目的本研究探讨了白花蛇舌草（Heracleum lasiopetalum Boiss）对醋酸诱导的 UC 大鼠模型的治疗效果。方法：48只成年雌性Wistar大鼠被随机分配到6组（每组8只），每组进行两次实验。通过结肠内注射 1 毫升 4% 乙酸 10 分钟诱导 UC。在治疗组中，雌性大鼠口服 5%、10% 和 40% 的植物水提取物、磺胺嘧啶（100 毫克/千克）和蒸馏水，为期 6 天，从注射醋酸 3 天后开始。保护组在施用醋酸前 7 天至施用醋酸后 3 天通过口服灌胃的方式接受药物。粘膜溃疡、充血、炎症和粘膜出血的程度根据杰拉尔德分类系统评分（宏观）进行评估。病理评估采用改良华莱士法（显微镜）在制备好的切片上进行。同时还检查了体重和结肠重量的变化，以及食物和水的摄入量。结果提取物组和阳性对照组的食物摄入量都发生了显著变化，与阴性对照组相比差异明显（P < 0.05）。与阴性对照组相比，阳性对照处理组的体重有明显增加（P < 0.05），而提取物处理组和阴性对照处理组之间没有明显差异（P > 0.05）。在水和食物摄入量以及体重变化方面，各保护组之间没有发现明显差异（P > 0.05）。显微镜和宏观检查均显示，与阴性对照组相比，提取物组和阳性对照组的结肠有明显改善（P < 0.05），各保护组之间无明显差异（P > 0.05）。结论千层塔提取物对减少结肠充血、粘膜溃疡、炎症和纤维化具有剂量依赖性。这凸显了其作为 UC 辅助治疗方案的潜力，强调了进一步研究的必要性。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.