Emerging Contributions of Pluripotent Stem Cells to Reproductive Technologies in Veterinary Medicine

IF 2.2 Q3 DEVELOPMENTAL BIOLOGY

Journal of Developmental Biology Pub Date : 2024-05-07 DOI:10.3390/jdb12020014

Raiane Cristina Fratini de Castro, Tiago William Buranello, K. Recchia, A. F. de Souza, N. C. G. Pieri, F. Bressan

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Abstract

The generation of mature gametes and competent embryos in vitro from pluripotent stem cells has been successfully achieved in a few species, mainly in mice, with recent advances in humans and scarce preliminary reports in other domestic species. These biotechnologies are very attractive as they facilitate the understanding of developmental mechanisms and stages that are generally inaccessible during early embryogenesis, thus enabling advanced reproductive technologies and contributing to the generation of animals of high genetic merit in a short period. Studies on the production of in vitro embryos in pigs and cattle are currently used as study models for humans since they present more similar characteristics when compared to rodents in both the initial embryo development and adult life. This review discusses the most relevant biotechnologies used in veterinary medicine, focusing on the generation of germ-cell-like cells in vitro through the acquisition of totipotent status and the production of embryos in vitro from pluripotent stem cells, thus highlighting the main uses of pluripotent stem cells in livestock species and reproductive medicine.

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多能干细胞对兽医生殖技术的新贡献

利用多能干细胞在体外生成成熟配子和合格胚胎的技术已在少数物种（主要是小鼠）中成功实现，最近在人类中也取得了进展，在其他家畜物种中也有少量初步报道。这些生物技术非常有吸引力，因为它们有助于了解早期胚胎发育过程中通常无法触及的发育机制和阶段，从而实现先进的生殖技术，并有助于在短期内产生高遗传性动物。猪和牛的体外胚胎生产研究目前被用作人类的研究模型，因为与啮齿类动物相比，猪和牛在胚胎初期发育和成年生活中都具有更相似的特征。本综述讨论了兽医学中使用的最相关的生物技术，重点是通过获得全能状态在体外生成类似生殖细胞的细胞，以及利用多能干细胞在体外生产胚胎，从而突出多能干细胞在家畜物种和生殖医学中的主要用途。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Developmental Biology Biochemistry, Genetics and Molecular Biology-Developmental Biology

CiteScore

4.10

自引率

18.50%

发文量

审稿时长

11 weeks

期刊介绍： The Journal of Developmental Biology (ISSN 2221-3759) is an international, peer-reviewed, quick-refereeing, open access journal, which publishes reviews, research papers and communications on the development of multicellular organisms at the molecule, cell, tissue, organ and whole organism levels. Our aim is to encourage researchers to effortlessly publish their new findings or concepts rapidly in an open access medium, overseen by their peers. There is no restriction on the length of the papers; the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material. Journal of Developmental Biology focuses on: -Development mechanisms and genetics -Cell differentiation -Embryonal development -Tissue/organism growth -Metamorphosis and regeneration of the organisms. It involves many biological fields, such as Molecular biology, Genetics, Physiology, Cell biology, Anatomy, Embryology, Cancer research, Neurobiology, Immunology, Ecology, Evolutionary biology.