Investigation of bioactive fractions from fenugreek seeds (Trigonella foenum-graecum L.): chemical profiling, in vitro anti-inflammatory activity, and their potential against LPS-stimulated J774A.1 cells

IF 1.8 Q3 PHARMACOLOGY & PHARMACY
Ensieh Kalantari, Mostafa Pournamdari, Mehrnaz Mehrabani, Mojdeh Esmaeili Tarzic, Neda Mohamadi, Amir Asadi, Sheida Illaghi, Fariba Sharififar
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引用次数: 0

Abstract

Despite the known anti-inflammatory effect of fenugreek seeds (Trigonella foenum-graecum L.), the specific mechanism and compounds responsible for this effect have been less studied so far. This research aimed to fractionate the total methanolic extract and investigate their impact on the iNOS expression in J774A.1 macrophage induced by lipopolysaccharide (LPS). The fractions of the total extract were obtained using semi-preparative HPLC. J774A.1 cells were treated with the highest non-toxic concentration of the samples and bacterial LPS (5 μg/ml). After 24 h, treated and untreated cells were used for RNA extraction, and mRNA levels were determined by qRT-PCR analysis. A total of 30 fractions were obtained with 9 main fractions (F3, F6, F9, F12, F15, F18, F21, F24, and F27) selected for cell studies. F9, total extract, and F15 significantly decreased iNOS gene expression compared to the LPS-treated group (up to 99.3%, 84.7% and 76.5% respectively). Overall, these results suggest that the anti-inflammatory effect of fenugreek seeds and/or fractions may be partially due to the suppression of iNOS. This effect can be attributed to various plant compounds, and further isolation may help identify novel anti-inflammatory compounds become a new inflammation. These findings support the potential of both the extract and the fractions as promising candidates for the treatment of inflammation-related diseases in future research.

葫芦巴种子(Trigonella foenum-graecum L.)中生物活性成分的研究:化学分析、体外抗炎活性及其对 LPS 刺激的 J774A.1 细胞的作用潜力
尽管已知葫芦巴籽(Trigonella foenum-graecum L.)具有抗炎作用,但迄今为止对其具体机制和化合物的研究较少。本研究旨在对甲醇提取物进行分馏,并研究其对脂多糖(LPS)诱导的 J774A.1 巨噬细胞中 iNOS 表达的影响。总提取物的馏分采用半制备高效液相色谱法获得。用最高无毒浓度的样品和细菌 LPS(5 μg/ml)处理 J774A.1 细胞。24 小时后,对处理过和未处理过的细胞进行 RNA 提取,并通过 qRT-PCR 分析测定 mRNA 水平。共获得 30 个馏分,其中 9 个主要馏分(F3、F6、F9、F12、F15、F18、F21、F24 和 F27)被选用于细胞研究。与 LPS 处理组相比,F9、总提取物和 F15 能明显降低 iNOS 基因的表达(分别高达 99.3%、84.7% 和 76.5%)。总之,这些结果表明,葫芦巴种子和/或馏分的抗炎作用可能部分是由于抑制了 iNOS。这种作用可归因于多种植物化合物,进一步分离可能有助于鉴定新型抗炎化合物,成为一种新的炎症。这些发现支持了提取物和馏分作为治疗炎症相关疾病的候选物质在未来研究中的潜力。
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来源期刊
Advances in Traditional Medicine
Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-
CiteScore
4.30
自引率
0.00%
发文量
50
期刊介绍: Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.
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