Selective apoptotic and genotoxic effects of pomegranate peel extract against human hepatocellular carcinoma HepG2 cell line

IF 1.8 Q3 PHARMACOLOGY & PHARMACY

Advances in Traditional Medicine Pub Date : 2024-05-15 DOI:10.1007/s13596-024-00753-2

Wesam T. Basal, Medhat El-Sakka, Neima K. El-Sonousy, Aliaa M. Issa

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Abstract

Finding a therapeutic agent that is selective, non-toxic to normal cells, and does not develop resistance or unpleasant side effects became the most challenging aspect in cancer therapy. Our study investigates the potential antitumorigenic effects of pomegranate peel extract against human hepatocellular carcinoma HepG₂ cell line. The effect of the extract on cell viability, DNA fragmentation, cell cycle arrest, and expression of cancer related genes in human hepatocellular carcinoma cell line (HepG₂), and normal human hepatocyte cell line (THLE₂) was evaluated using MTT, comet assay, flow cytometry and real time PCR, respectively. Unlike THLE₂ cells, the pomegranate peel extract significantly decreased cell viability accompanied by DNA fragmentation and cell cycle arrest in HepG₂ cells. It also significantly decreased the expression levels of Cyclooxygenase-2, B-cell lymphoma 2, vascular permeability factor, matrix metalloproteinase -9, cyclin B1, and cyclin dependent kinase1 while the expression levels of BCL2-associated X protein, and p21 genes were significantly increased. The obtained results suggest a potential antitumorigenic effect of pomegranate peel extract against HepG₂ cell lines.

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石榴皮提取物对人肝癌 HepG2 细胞系的选择性凋亡和基因毒性作用

寻找一种选择性强、对正常细胞无毒、不会产生抗药性或令人不快的副作用的治疗剂成为癌症治疗中最具挑战性的问题。我们的研究调查了石榴皮提取物对人类肝癌 HepG2 细胞系的潜在抗肿瘤作用。研究使用 MTT、彗星试验、流式细胞仪和实时 PCR 分别评估了石榴皮提取物对人肝癌细胞系（HepG2）和正常人肝癌细胞系（THLE2）的细胞活力、DNA 断裂、细胞周期停滞和癌症相关基因表达的影响。与 THLE2 细胞不同的是，石榴皮提取物能显著降低 HepG2 细胞的存活率，并伴有 DNA 断裂和细胞周期停滞。石榴皮提取物还能明显降低环氧化酶-2、B 细胞淋巴瘤 2、血管通透性因子、基质金属蛋白酶-9、细胞周期蛋白 B1 和细胞周期蛋白依赖性激酶 1 的表达水平，而 BCL2 相关 X 蛋白和 p21 基因的表达水平则明显升高。这些结果表明，石榴皮提取物对 HepG2 细胞株具有潜在的抗肿瘤作用。

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来源期刊

Advances in Traditional Medicine PHARMACOLOGY & PHARMACY-

CiteScore

4.30

自引率

0.00%

发文量

期刊介绍： Advances in Traditional Medicine (ADTM) is an international and peer-reviewed journal and publishes a variety of articles including original researches, reviews, short communications, and case-reports. ADTM aims to bridging the gap between Traditional knowledge and medical advances. The journal focuses on publishing valid, relevant, and rigorous experimental research and clinical applications of Traditidnal Medicine as well as medical classics. At the same time, the journal is devoted to communication among basic researcher and medical clinician interested in the advancement of Traditional Medicine. Topics covered by the journal are: Medical Classics & History; Biomedical Research; Pharmacology & Toxicology of Natural Products; Acupuncture & Moxibustion; Sasang Constitutional Medicine; Diagnostics and Instrumental Development; Clinical Research. ADTM is published four times yearly. The publication date of this journal is 30th March, June, September, and December.