Enhancing Secondary Metabolite Production in Pelargonium graveolens Hort. Cell Cultures: Eliciting Effects of Chitosan and Jasmonic Acid on Bioactive Compound Production
IF 4.3 3区 材料科学Q1 ENGINEERING, ELECTRICAL & ELECTRONIC
Amine Elbouzidi, Mohamed Taibi, Abdellah Baraich, Mounir Haddou, E. Loukili, A. Asehraou, François Mesnard, M. Addi
{"title":"Enhancing Secondary Metabolite Production in Pelargonium graveolens Hort. Cell Cultures: Eliciting Effects of Chitosan and Jasmonic Acid on Bioactive Compound Production","authors":"Amine Elbouzidi, Mohamed Taibi, Abdellah Baraich, Mounir Haddou, E. Loukili, A. Asehraou, François Mesnard, M. Addi","doi":"10.3390/horticulturae10050521","DOIUrl":null,"url":null,"abstract":"This study explores the effects of chitosan (CHT) and jasmonic acid (JA) elicitors on rose-scented geranium (Pelargonium graveolens Hort.) cell suspension cultures, aiming to enhance the production of phenolics and flavonoids and antioxidant properties. Elicitation with CHT and JA resulted in varied biomass yields and callus characteristics, with higher concentrations generally leading to increased phenolic accumulation. Optimal biomass was achieved with CHT4 (75 mg/mL) and JA3 (50 µM) treatments. HPLC-DAD analysis revealed changes in phenolic compound composition and quantities, with specific compounds induced by either CHT4 or JA3. For instance, gallic acid content increased significantly in CHT4-treated cells, while catechin content increased notably in both CHT4 and JA3 treatments. Antioxidant enzyme activities like superoxide dismutase and peroxidase increased with elicitor concentration, particularly in CHT4 and JA3 treatments. Both treatments exhibited potent antioxidant activity, with JA3 exhibiting the lowest IC50 value in the DPPH assay and highest total antioxidant capacity (TAC) values. Surprisingly, both CHT4 and JA3 extracts effectively inhibited tyrosinase activity. These findings underscore the efficacy of CHT and JA elicitors in enhancing phenolic and flavonoid production, boosting antioxidant capacity, and inhibiting tyrosinase activity in P. graveolens cultures, offering promising implications for further research and industrial applications in pharmaceutical and cosmetic sectors.","PeriodicalId":3,"journal":{"name":"ACS Applied Electronic Materials","volume":"3 4","pages":""},"PeriodicalIF":4.3000,"publicationDate":"2024-05-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ACS Applied Electronic Materials","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/horticulturae10050521","RegionNum":3,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENGINEERING, ELECTRICAL & ELECTRONIC","Score":null,"Total":0}
引用次数: 0
Abstract
This study explores the effects of chitosan (CHT) and jasmonic acid (JA) elicitors on rose-scented geranium (Pelargonium graveolens Hort.) cell suspension cultures, aiming to enhance the production of phenolics and flavonoids and antioxidant properties. Elicitation with CHT and JA resulted in varied biomass yields and callus characteristics, with higher concentrations generally leading to increased phenolic accumulation. Optimal biomass was achieved with CHT4 (75 mg/mL) and JA3 (50 µM) treatments. HPLC-DAD analysis revealed changes in phenolic compound composition and quantities, with specific compounds induced by either CHT4 or JA3. For instance, gallic acid content increased significantly in CHT4-treated cells, while catechin content increased notably in both CHT4 and JA3 treatments. Antioxidant enzyme activities like superoxide dismutase and peroxidase increased with elicitor concentration, particularly in CHT4 and JA3 treatments. Both treatments exhibited potent antioxidant activity, with JA3 exhibiting the lowest IC50 value in the DPPH assay and highest total antioxidant capacity (TAC) values. Surprisingly, both CHT4 and JA3 extracts effectively inhibited tyrosinase activity. These findings underscore the efficacy of CHT and JA elicitors in enhancing phenolic and flavonoid production, boosting antioxidant capacity, and inhibiting tyrosinase activity in P. graveolens cultures, offering promising implications for further research and industrial applications in pharmaceutical and cosmetic sectors.
期刊介绍:
ACS Applied Electronic Materials is an interdisciplinary journal publishing original research covering all aspects of electronic materials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials science, engineering, optics, physics, and chemistry into important applications of electronic materials. Sample research topics that span the journal's scope are inorganic, organic, ionic and polymeric materials with properties that include conducting, semiconducting, superconducting, insulating, dielectric, magnetic, optoelectronic, piezoelectric, ferroelectric and thermoelectric.
Indexed/Abstracted:
Web of Science SCIE
Scopus
CAS
INSPEC
Portico