Towards a portable setup for the on-site SERS detection of miRNAs

IF 1.9 4区物理与天体物理 Q3 OPTICS

Journal of the European Optical Society-Rapid Publications Pub Date : 2024-05-20 DOI:10.1051/jeos/2024028

Chiara Novara

引用次数: 0

Abstract

The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by Surface Enhanced Raman Scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.

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开发现场 SERS 检测 miRNA 的便携式装置

对 miRNA 等生物标记物进行现场分析的实际实施取决于是否有便携式、易于操作的检测系统，可以在实际生活中作为护理点使用。在这项工作中，报告了一种与便携式拉曼光谱仪相结合的自动化微流控平台，并通过表面增强拉曼散射（SERS）对 miR-214 进行了检测。根据先前优化的两步杂交测定法功能化的多腔 SERS 活性芯片与分析溶液一起自动孵育。通过初步测试，可以调整便携式拉曼光谱仪的采集条件，并将获得的结果与台式拉曼显微镜的结果进行比较。最后，通过自动程序分析了不同浓度的 miR-214，检测限（LOD）达到了皮摩尔范围。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the European Optical Society-Rapid Publications 物理-光学

CiteScore

2.40

自引率

0.00%

发文量

审稿时长

5 weeks

期刊介绍： Rapid progress in optics and photonics has broadened its application enormously into many branches, including information and communication technology, security, sensing, bio- and medical sciences, healthcare and chemistry. Recent achievements in other sciences have allowed continual discovery of new natural mysteries and formulation of challenging goals for optics that require further development of modern concepts and running fundamental research. The Journal of the European Optical Society – Rapid Publications (JEOS:RP) aims to tackle all of the aforementioned points in the form of prompt, scientific, high-quality communications that report on the latest findings. It presents emerging technologies and outlining strategic goals in optics and photonics. The journal covers both fundamental and applied topics, including but not limited to: Classical and quantum optics Light/matter interaction Optical communication Micro- and nanooptics Nonlinear optical phenomena Optical materials Optical metrology Optical spectroscopy Colour research Nano and metamaterials Modern photonics technology Optical engineering, design and instrumentation Optical applications in bio-physics and medicine Interdisciplinary fields using photonics, such as in energy, climate change and cultural heritage The journal aims to provide readers with recent and important achievements in optics/photonics and, as its name suggests, it strives for the shortest possible publication time.