Towards a portable setup for the on-site SERS detection of miRNAs

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-20 DOI:10.1051/jeos/2024028

Chiara Novara

引用次数: 0

Abstract

The actual implementation of on-site analysis of biomarkers, such as miRNAs, depends on the availability of portable and easy-to-handle detection systems that can be used as point-of-care in real life applications. In this work, an automatized microfluidic platform combined with a portable Raman spectrometer is reported and tested for miR-214 detection by Surface Enhanced Raman Scattering (SERS). A multi-chamber SERS-active chip, functionalized according to a previously optimized two-step hybridization assay, was automatically incubated with the analyte solution. Preliminary tests allowed to adjust the portable Raman spectrometer acquisition conditions and to compare the obtained results with those of a bench Raman microscope. Finally, miR-214 at different concentrations was analyzed through an automatic procedure, achieving a limit of detection (LOD) in the picomolar range.

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开发现场 SERS 检测 miRNA 的便携式装置

对 miRNA 等生物标记物进行现场分析的实际实施取决于是否有便携式、易于操作的检测系统，可以在实际生活中作为护理点使用。在这项工作中，报告了一种与便携式拉曼光谱仪相结合的自动化微流控平台，并通过表面增强拉曼散射（SERS）对 miR-214 进行了检测。根据先前优化的两步杂交测定法功能化的多腔 SERS 活性芯片与分析溶液一起自动孵育。通过初步测试，可以调整便携式拉曼光谱仪的采集条件，并将获得的结果与台式拉曼显微镜的结果进行比较。最后，通过自动程序分析了不同浓度的 miR-214，检测限（LOD）达到了皮摩尔范围。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.